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Literature summary for 1.1.1.195 extracted from

  • Gabotti, D.; Negrini, N.; Morgutti, S.; Nocito, F.F.; Cocucci, M.
    Cinnamyl alcohol dehydrogenases in the mesocarp of ripening fruit of Prunus persica genotypes with different flesh characteristics: changes in activity and protein and transcript levels (2015), Physiol. Plant., 154, 329-348.
    View publication on PubMed
Search for more literature for 1.1.1.195

Cloned(Commentary)

Cloned (Comment) Organism
gene CAD, isozyme genotyping, phylogenetic analysis, sequence comparisons, and expression analysis of isozymes Prunus persica

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, Zn1-binding motif [GHE(X)2G(X)5G(X)2 V] and Zn2-binding motif [GD(X)9,10C(X)2C(X)2C(X)7C] occur in most of the isozymes, overview Prunus persica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coniferyl alcohol + NADP+ Prunus persica
-
 coniferyl aldehyde + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Prunus persica
-
 yellow-fleshed NMF Oro A and MF Springcrest, and blood-flesh MF Sanguinella
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit high levels of CAD activity/expression in Sanguinella, a blood flesh cultivar, phenolics and lignin contents and CAD activities, overview Prunus persica
-
mesocarp
-
 Prunus persica
-
additional information in silico analysis of CAD genes Prunus persica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coniferyl alcohol + NADP+
-
 Prunus persica coniferyl aldehyde + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
CAD
-
 Prunus persica
cinnamyl alcohol dehydrogenases
-
 Prunus persica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Prunus persica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 coniferyl alcohol oxidation assay at Prunus persica

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Prunus persica
NADPH the NADPH-binding motif [GXG(X)2G] is present in all isoforms Prunus persica

General Information

General Information Comment Organism
evolution a total of 24 putative full-length PRUPE_CAD genes are identified (in silico analysis) in the peach genome, overview Prunus persica
physiological function the enzyme CAD activity is related to neither lignification nor differences in flesh firmness in the cultivars non-melting flesh Oro A/melting flesh Springcrest and Sanguinella, and color, blood-flesh Sanguinella, but might play a role in fruit ripening Prunus persica