Inhibitors | Comment | Organism | Structure |
---|---|---|---|
caffeic acid | - |
Leucaena leucocephala | |
ferulic acid | - |
Leucaena leucocephala | |
additional information | no inhibition by salicylic acid | Leucaena leucocephala | |
Zn2+ | inhibitory at 2.0 mM, Zn2+ treatment leads to the formation of a bigger oligomer of CAD which is catalytically inactive. EDTA treatment prevents the formation of the bigger CAD oligomer and hence increases the CAD activity | Leucaena leucocephala |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
NADPH | pH 6.5, 40°C | Leucaena leucocephala | |
0.0015 | - |
cinnamaldehyde | pH 6.5, 40°C | Leucaena leucocephala | |
0.0038 | - |
cinnamyl alcohol | pH 8.8, 40°C | Leucaena leucocephala | |
0.0045 | - |
coniferyl aldehyde | pH 6.5, 40°C | Leucaena leucocephala | |
0.005 | - |
coniferyl alcohol | pH 8.8, 40°C | Leucaena leucocephala | |
0.0062 | - |
sinapyl alcohol | pH 8.8, 40°C | Leucaena leucocephala | |
0.0085 | - |
NADP+ | pH 8.8, 40°C | Leucaena leucocephala |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Leucaena leucocephala | 5618 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
EDTA | activates at 2.0 mM, Zn2+ treatment leads to the formation of a bigger oligomer of CAD which is catalytically inactive. EDTA treatment prevents the formation of the bigger CAD oligomer and hence increases the CAD activity | Leucaena leucocephala | |
Mg2+ | 30% activating at 2.0 mM | Leucaena leucocephala | |
additional information | Ca2+ shows no significant effect | Leucaena leucocephala | |
Zn2+ | dependent on, zinc-dependent metalloenzyme | Leucaena leucocephala |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Leucaena leucocephala |
76000 | - |
gel filtration | Leucaena leucocephala |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamaldehyde + NADPH + H+ | Leucaena leucocephala | - |
cinnamyl alcohol + NADP+ | - |
r | |
coniferyl aldehyde + NADPH + H+ | Leucaena leucocephala | the forward reaction is the physiological reaction | coniferyl alcohol + NADP+ | - |
r | |
sinapyl aldehyde + NADPH + H+ | Leucaena leucocephala | - |
sinapyl alcohol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucaena leucocephala | - |
cv. K-29, from the garden of Department of Biochemistry, Lucknow University, India | - |
Purification (Comment) | Organism |
---|---|
native enzyme 514.2fold from secondary xylem by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and affinity chromatography (elution with NADP+) | Leucaena leucocephala |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Leucaena leucocephala | - |
xylem | secondary | Leucaena leucocephala | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48.74 | - |
purified native enzyme, pH 8.8, 40°C | Leucaena leucocephala |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamaldehyde + NADPH + H+ | - |
Leucaena leucocephala | cinnamyl alcohol + NADP+ | - |
r | |
cinnamyl alcohol + NADP+ | - |
Leucaena leucocephala | cinnamaldehyde + NADPH + H+ | - |
r | |
coniferyl alcohol + NADP+ | - |
Leucaena leucocephala | coniferyl aldehyde + NADPH + H+ | - |
r | |
coniferyl aldehyde + NADPH + H+ | - |
Leucaena leucocephala | coniferyl alcohol + NADP+ | - |
r | |
coniferyl aldehyde + NADPH + H+ | the forward reaction is the physiological reaction | Leucaena leucocephala | coniferyl alcohol + NADP+ | - |
r | |
additional information | no activity with methanol and ethanol as alcoholic substrates | Leucaena leucocephala | ? | - |
? | |
p-coumaryl alcohol + NADP+ | - |
Leucaena leucocephala | p-coumaryl aldehyde + NADPH + H+ | - |
r | |
sinapyl alcohol + NADP+ | - |
Leucaena leucocephala | sinapyl aldehyde + NADPH + H+ | - |
r | |
sinapyl aldehyde + NADPH + H+ | - |
Leucaena leucocephala | sinapyl alcohol + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38000, SDS-PAGE | Leucaena leucocephala |
Synonyms | Comment | Organism |
---|---|---|
CAD | - |
Leucaena leucocephala |
cinnamyl alcohol dehydrogenase | - |
Leucaena leucocephala |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Leucaena leucocephala |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 42 | good activity between 30°C to 42°C with maximum activity at 40°C, beyond which activity declined sharply | Leucaena leucocephala |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.63 | - |
cinnamaldehyde | pH 6.5, 40°C | Leucaena leucocephala | |
6.41 | - |
cinnamyl alcohol | pH 8.8, 40°C | Leucaena leucocephala | |
8.75 | - |
coniferyl alcohol | pH 8.8, 40°C | Leucaena leucocephala | |
9.22 | - |
NADP+ | pH 8.8, 40°C | Leucaena leucocephala | |
14.38 | - |
sinapyl alcohol | pH 8.8, 40°C | Leucaena leucocephala | |
15.31 | - |
NADPH | pH 6.5, 40°C | Leucaena leucocephala | |
16.56 | - |
coniferyl aldehyde | pH 6.5, 40°C | Leucaena leucocephala |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
coniferyl aldehyde reduction | Leucaena leucocephala |
8.8 | - |
coniferyl alcohol oxidation | Leucaena leucocephala |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Leucaena leucocephala | |
NADPH | - |
Leucaena leucocephala |
General Information | Comment | Organism |
---|---|---|
evolution | purified CAD by MALDI-TOF shows a significant homology to alcohol dehydrogenases of MDR superfamily | Leucaena leucocephala |
physiological function | cinnamyl alcohol dehydrogenase catalyzes the reversible conversion of hydroxycinnamyl aldehydes to their corresponding alcohols, before their oxidative polymerization to lignin, a major constituent of the plant cell wall | Leucaena leucocephala |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1090 | - |
NADP+ | pH 8.8, 40°C | Leucaena leucocephala | |
1690 | - |
cinnamyl alcohol | pH 8.8, 40°C | Leucaena leucocephala | |
1750 | - |
coniferyl alcohol | pH 8.8, 40°C | Leucaena leucocephala | |
2320 | - |
sinapyl alcohol | pH 8.8, 40°C | Leucaena leucocephala | |
3680 | - |
coniferyl aldehyde | pH 6.5, 40°C | Leucaena leucocephala | |
3750 | - |
cinnamaldehyde | pH 6.5, 40°C | Leucaena leucocephala | |
10210 | - |
NADPH | pH 6.5, 40°C | Leucaena leucocephala |