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Literature summary for 1.1.1.195 extracted from
- Expression pattern of two paralogs encoding cinnamyl alcohol dehydrogenases in Arabidopsis. Isolation and characterization of the corresponding mutants (2003), Plant Physiol., 132, 848-860.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 9 AtCAD genes, 2 paralogs AtCAD-C and AtCAD-D, DNA and amino acid sequence determination and analysis, tissue expression pattern study of wild-type and mutant plants | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of null-mutants of both genes AtCAD-C and AtCAD-D showing highly reduced enzyme activity, but only the latter shows slightly modificated lignin structure, construction of transgenic plants by infection with recombinant Agrobacterium tumefaciens strain C58pMP90 via flower infiltration method, expression pattern of AtCAD-C and AtCAD-D | Arabidopsis thaliana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
Arabidopsis thaliana |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, AtCAD-C, SDS-PAGE, x * 44000, AtCAD-D, SDS-PAGE | Arabidopsis thaliana |
| 44000 | - |
x * 42000, AtCAD-C, SDS-PAGE, x * 44000, AtCAD-D, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferol + NADP+ | Arabidopsis thaliana | - |
coniferyl aldehyde + NADPH | - |
? | |
| additional information | Arabidopsis thaliana | role of the enzyme in lignin biosynthesis and structure formation | ? | - |
? | |
| sinapyl alcohol + NADP+ | Arabidopsis thaliana | - |
sinapyl aldehyde + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q02971 | 9 AtCAD genes, 2 paralogs AtCAD-C and AtCAD-D | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| additional information | enzyme expression pattern of AtCAD-C and AtCAD-D, no expression in silique | Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| stem | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferol + NADP+ | - |
Arabidopsis thaliana | coniferyl aldehyde + NADPH | - |
? | |
| additional information | role of the enzyme in lignin biosynthesis and structure formation | Arabidopsis thaliana | ? | - |
? | |
| sinapyl alcohol + NADP+ | - |
Arabidopsis thaliana | sinapyl aldehyde + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, AtCAD-C, SDS-PAGE, x * 44000, AtCAD-D, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CAD | - |
Arabidopsis thaliana |
| cinnamyl alcohol dehydrogenase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
assay at | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Arabidopsis thaliana | |
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