Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.195 extracted from

  • O'Malley, D.M.; Porter, S.; Sederoff, R.R.
    Purification, characterization, and cloning of cinnamyl alcohol dehydrogenase in loblolly pine (Pinus taeda L.) (1992), Plant Physiol., 98, 1364-1371.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Pinus taeda

Inhibitors

Inhibitors Comment Organism Structure
coniferin with coniferyl alcohol as substrates, noncompetitive inhibition Pinus taeda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
coniferaldehyde
-
Pinus taeda
0.0046
-
NADPH with coniferaldehyde Pinus taeda
0.0053
-
cinnamaldehyde
-
Pinus taeda
0.0067
-
NADPH with cinnamaldehyde Pinus taeda
0.147
-
sinapaldehyde
-
Pinus taeda

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
2 * 44000, SDS-PAGE Pinus taeda
82000
-
gel filtration (on Sephacryl 200) Pinus taeda

Organism

Organism UniProt Comment Textmining
Pinus taeda
-
loblolly pine
-

Purification (Commentary)

Purification (Comment) Organism
-
Pinus taeda

Source Tissue

Source Tissue Comment Organism Textmining
seed developing Pinus taeda
-
xylem differentiating tissue Pinus taeda
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
crude extract from differentiating xylem Pinus taeda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cinnamyl alcohol + NADP+
-
Pinus taeda cinnamaldehyde + NADPH
-
r
coniferyl alcohol + NADP+
-
Pinus taeda coniferyl aldehyde + NADPH + H+
-
r
sinapyl alcohol + NADP+
-
Pinus taeda sinapaldehyde + NADPH
-
r

Subunits

Subunits Comment Organism
dimer 2 * 44000, SDS-PAGE Pinus taeda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
coniferaldehyde reduction Pinus taeda
9
-
coniferyl alcohol oxidation dependent on ionic strength Pinus taeda

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Pinus taeda