General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Populus x canadensis |
60% loss of activity after 20 h incubation in buffer containing a divalent cation-exchange resin | Populus x canadensis |
dithiothreitol stabilizes | Populus x canadensis |
ethylene glycol stabilizes | Populus x canadensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00077 | - |
coniferaldehyde | - |
Populus x canadensis | |
0.0012 | - |
p-coumaraldehyde | - |
Populus x canadensis | |
0.0048 | - |
sinapaldehyde | - |
Populus x canadensis | |
0.008 | - |
NADPH | - |
Populus x canadensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | restores activity after incubation in buffer containing a divalent-cation exchange resin | Populus x canadensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Populus x canadensis |
70000 | - |
gel filtration | Populus x canadensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus x canadensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Populus x canadensis |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.71 | - |
- |
Populus x canadensis |
Storage Stability | Organism |
---|---|
4°C, mercaptoethanol, ethylene glycol, 1 month | Populus x canadensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coniferyl alcohol + NADP+ | - |
Populus x canadensis | coniferyl aldehyde + NADPH + H+ | - |
? | |
p-coumaryl alcohol + NADP+ | - |
Populus x canadensis | p-coumaraldehyde + NADPH | - |
? | |
sinapyl alcohol + NADP+ | - |
Populus x canadensis | sinapaldehyde + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Populus x canadensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of coniferyl aldehyde, KH2PO4/Na2HPO4 buffer | Populus x canadensis |
7 | - |
reduction of coniferyl aldehyde, Tris maleate/NaOH buffer | Populus x canadensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
4°C, 1 week, 50% loss of activity | Populus x canadensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Populus x canadensis |