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Literature summary for 1.1.1.191 extracted from
- Isolation and characterization of indole-3-acetaldehyde reductases from Cucumis sativus (1976), J. Biol. Chem., 251, 907-913.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | activation up to 0.1 M | Cucumis sativus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
Indole-3-acetaldehyde | at pH 7.0, isoenzyme TH | Cucumis sativus | |
| 0.133 | - |
Indole-3-acetaldehyde | at pH 7.0, isoenzyme TL | Cucumis sativus | |
| 0.2 | - |
Indole-3-acetaldehyde | at pH 5.2, isoenzyme TH | Cucumis sativus | |
| 0.25 | - |
Indole-3-acetaldehyde | at pH 5.2, isoenzyme TL | Cucumis sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
isoenzyme TL, gel filtration | Cucumis sativus |
| 52000 | - |
isoenzyme TH, gel filtration | Cucumis sativus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| indole-3-acetaldehyde + NADPH | Cucumis sativus | - |
indole-3-ethanol + NADP+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Cucumis sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | two isoenzymes, TH and TL | Cucumis sativus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NADPH + H+ | very low activity only for isoenzyme TH | Cucumis sativus | ethanol + NADP+ | - |
? | |
| butyraldehyde + NADPH | low activity | Cucumis sativus | butanol + NADP+ | - |
? | |
| indole-3-acetaldehyde + NADPH | - |
Cucumis sativus | indole-3-ethanol + NADP+ | - |
ir | |
| phenylacetone + NADPH | low activity | Cucumis sativus | phenylethanol + NADP+ | - |
? | |
| propionaldehyde + NADPH | very low activity only for isoenzyme TL | Cucumis sativus | propanol + NADP+ | - |
? | |
| trans-cinnamaldehyde + NADPH | very low activity | Cucumis sativus | (2E)-3-phenylprop-2-en-1-ol + NADP+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
second pH optimum at pH 7.0 | Cucumis sativus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Cucumis sativus | |
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