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Literature summary for 1.1.1.190 extracted from
- Indoleacetaldehyde reductase of Cucumis sativus L. Kinetic properties and role in auxin biosynthesis (1980), Plant Physiol., 65, 107-113.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | complete inhibition at 2 mM | Cucumis sativus |  |
| Cu2+ | complete inhibition at 2 mM | Cucumis sativus | |
| EDTA | strong inhibition at 10 mM | Cucumis sativus | |
| Hg2+ | complete inhibition at 2 mM | Cucumis sativus | |
| iodoacetic acid | 62% inhibition at 10 mM | Cucumis sativus | |
| KCN | complete inhibition at 1 mM | Cucumis sativus | |
| N-ethylmaleimide | 8§% inhibition at 1 mM | Cucumis sativus | |
| NaCl | uncompetitive inhibition with respect to NADH, competitive inhibition with respect to indole-3-acetaldehyde | Cucumis sativus | |
| NaN3 | slight inhibition at 10 mM | Cucumis sativus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
NADH | marked function of the NaCl concentration | Cucumis sativus | |
| 0.5 | - |
Indole-3-acetaldehyde | marked function of the NaCl concentration | Cucumis sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
gel filtration | Cucumis sativus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| indole-3-acetaldehyde + NADH | Cucumis sativus | involved in auxin biosynthetic pathway | indole-3-ethanol + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Cucumis sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Cucumis sativus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| indole-3-acetaldehyde + NADH | involved in auxin biosynthetic pathway | Cucumis sativus | indole-3-ethanol + NAD+ | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Cucumis sativus | |
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