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Literature summary for 1.1.1.185 extracted from

  • Carballo, J.; Bernardo, A.; Prieto, M.J.G.; Sarmiento, R.M.
    Kinetics of alpha-dicarbonyls reduction by L-glycol dehydrogenase (NAD+) from Enterobacter aerogenes (1993), Ital. J. Biochem., 42, 79-89.
    View publication on PubMed
Search for more literature for 1.1.1.185

Inhibitors

Inhibitors Comment Organism Structure
acetone uncompetitive for NADH Klebsiella aerogenes
L-acetoin probably all L(+)-alpha-hydroxycarbonyls products inhibit uncompetitively at non-saturated concentrations Klebsiella aerogenes
NAD+ competitive inhibition for NADH Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 NADH with ethyl pyruvate Klebsiella aerogenes
0.0062
-
 NADH with 2,3-pentadione Klebsiella aerogenes
0.0088
-
 NADH with diacetyl Klebsiella aerogenes
0.0097
-
 NADH with methylglyoxal Klebsiella aerogenes
1.5
-
 diacetyl
-
 Klebsiella aerogenes
10
-
 2,3-Pentanedione
-
 Klebsiella aerogenes
22
-
 ethyl pyruvate
-
 Klebsiella aerogenes
90
-
 methylglyoxal
-
 Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Reaction

Reaction Comment Organism Reaction ID
an L-glycol + NAD(P)+ = a 2-hydroxycarbonyl compound + NAD(P)H + H+ ordered bi-bi-mechanism, coenzyme binds as first substrate to the enzyme Klebsiella aerogenes
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-pentanedione + NADH
-
 Klebsiella aerogenes L-acetylethylcarbinol + NAD+
-
 ir
diacetyl + NADH
-
 Klebsiella aerogenes L-acetoin + NAD+
-
 ir
ethyl pyruvate + NAD(P)H
-
 Klebsiella aerogenes ethyl lactate
-
 ?
methylglyoxal + NADH
-
 Klebsiella aerogenes L-lactaldehyde
-
 ir
R1-CO-CHOH-R2 + NAD(P)H 2-hydroxycarbonyl compound e.g. glycolaldehyde, glyceraldehyde, acetoin, acetylethylcarbinol, diacetylmethylcarbinol Klebsiella aerogenes R1-CHOH-CHOH-R2 + NAD(P)+
-
 r
R1-CO-CHOH-R2 + NAD(P)H much more efficient in dehydrogenase direction Klebsiella aerogenes R1-CHOH-CHOH-R2 + NAD(P)+
-
 r
R1-CO-CO-R2 + NAD(P)H methylglyoxal, diacetyl, 2,3-pentanedione Klebsiella aerogenes R1-CO-CHOH-R2 + NAD(P)+
-
 ir

Synonyms

Synonyms Comment Organism
L-glycol dehydrogenase (NAD+)
-
 Klebsiella aerogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Klebsiella aerogenes
NADH
-
 Klebsiella aerogenes