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Literature summary for 1.1.1.184 extracted from

  • Karanam, V.; Chaudhury, D.; Chadha, A.
    Understanding (R) specific carbonyl reductase from candida parapsilosis ATCC 7330 [CpCR] Substrate scope, kinetic studies and the role of zinc (2019), Catalysts, 9, 702 .
No PubMed abstract available
Search for more literature for 1.1.1.184

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Candida parapsilosis
Cu2+ strong inhibition Candida parapsilosis
EDTA addition of EDTA to the enzyme solution in a 1:3 enzyme: EDTA mole ratio, results in a drop of the specific activity of the enzyme immediately by 30% Candida parapsilosis
Mg2+
-
 Candida parapsilosis
Mn2+
-
 Candida parapsilosis
Ni2+
-
 Candida parapsilosis
ZnCl2
-
 Candida parapsilosis
ZnSO4
-
 Candida parapsilosis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains two different Zn atoms viz. catalytic Zn and the structural Zn Candida parapsilosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration Candida parapsilosis

Organism

Organism UniProt Comment Textmining
Candida parapsilosis M4VRJ6
-
-
Candida parapsilosis ATCC 7330 M4VRJ6
-
-

Purification (Commentary)

Purification (Comment) Organism
GST column chromatography and Superdex gel filtration Candida parapsilosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8
-
 with 2,4-dichlorobenzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis
6.53
-
 with 3-fluorobenzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis
6.76
-
 with 2-bromobenzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis
13.35
-
 with 4-nitrobenzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis
18.63
-
 with 4-cyanobenzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis
19.29
-
 with pentanal as substrate, at pH 7.5 and 30°C Candida parapsilosis
19.69
-
 with 4-methoxybenzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis
20.79
-
 with 3-bromobenzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis
21
-
 with 4-bromobenzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis
21.17
-
 with 2-cyanobenzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis
21.9
-
 with hexanal as substrate, at pH 7.5 and 30°C Candida parapsilosis
23.06
-
 with 2-fluorobenzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis
24.98
-
 with propanal as substrate, at pH 7.5 and 30°C Candida parapsilosis
26.46
-
 with 4-methylbenzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis
33.96
-
 with benzaldehyde as substrate, at pH 7.5 and 30°C Candida parapsilosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dichlorobenzaldehyde + NADPH + H+ lowest activity Candida parapsilosis 2,4-dichlorobenzyl alcohol + NADP+
-
 ?
2-bromobenzaldehyde + NADPH + H+
-
 Candida parapsilosis 2-bromobenzyl alcohol + NADP+
-
 ?
2-cyanobenzaldehyde + NADPH + H+
-
 Candida parapsilosis 2-cyanobenzyl alcohol + NADP+
-
 ?
2-fluorobenzaldehyde + NADPH + H+
-
 Candida parapsilosis 2-fluorobenzyl alcohol + NADP+
-
 ?
3-bromobenzaldehyde + NADPH + H+
-
 Candida parapsilosis 3-bromobenzyl alcohol + NADP+
-
 ?
3-fluorobenzaldehyde + NADPH + H+
-
 Candida parapsilosis 3-fluorobenzyl alcohol + NADP+
-
 ?
4-bromobenzaldehyde + NADPH + H+
-
 Candida parapsilosis 4-bromobenzyl alcohol + NADP+
-
 ?
4-cyanobenzaldehyde + NADPH + H+
-
 Candida parapsilosis 4-cyanobenzyl alcohol + NADP+
-
 ?
4-methoxybenzaldehyde + NADPH + H+
-
 Candida parapsilosis 4-methoxybenzyl alcohol + NADP+
-
 ?
4-methoxybenzaldehyde + NADPH + H+
-
 Candida parapsilosis ATCC 7330 4-methoxybenzyl alcohol + NADP+
-
 ?
4-methylbenzaldehyde + NADPH + H+
-
 Candida parapsilosis 4-methylbenzyl alcohol + NADP+
-
 ?
4-nitrobenzaldehyde + NADPH + H+
-
 Candida parapsilosis 4-nitrobenzyl alcohol + NADP+
-
 ?
4-nitrobenzaldehyde + NADPH + H+
-
 Candida parapsilosis ATCC 7330 4-nitrobenzyl alcohol + NADP+
-
 ?
benzaldehyde + NADPH + H+ highest activity Candida parapsilosis benzyl alcohol + NADP+
-
 ?
benzaldehyde + NADPH + H+ highest activity Candida parapsilosis ATCC 7330 benzyl alcohol + NADP+
-
 ?
hexanal + NADPH + H+
-
 Candida parapsilosis hexan-1-ol + NADP+
-
 ?
pentanal + NADPH + H+
-
 Candida parapsilosis pentan-1-ol + NADP+
-
 ?
pentanal + NADPH + H+
-
 Candida parapsilosis ATCC 7330 pentan-1-ol + NADP+
-
 ?
propanal + NADPH + H+
-
 Candida parapsilosis propan-1-ol + NADP+
-
 ?
propanal + NADPH + H+
-
 Candida parapsilosis ATCC 7330 propan-1-ol + NADP+
-
 ?

Subunits

Subunits Comment Organism
heterodimer
-
 Candida parapsilosis

Synonyms

Synonyms Comment Organism
(R) specific carbonyl reductase
-
 Candida parapsilosis