Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.184 extracted from

  • Jin, Q.; Wu, Z.; Dou, Y.; Yang, Y.; Xia, J.; Jin, Z.
    A novel carbonyl reductase with anti-Prelog stereospecificity for the production of t-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate (2019), 3 Biotech, 9, 194 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells [Candida] boidinii

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile 37.7% residual activity at 5% (v/v) [Candida] boidinii
Cu2+ 7.2 % residual activity at 5 mM [Candida] boidinii
dimethyl sulfoxide 32.6% residual activity at 5% (v/v) [Candida] boidinii
EDTA 89.8% residual activity at 5 mM [Candida] boidinii
ethanol 73.6% residual activity at 5% (v/v) [Candida] boidinii
Fe3+ 2.2 % residual activity at 5 mM [Candida] boidinii
Isopropanol 41.1% residual activity at 5% (v/v) [Candida] boidinii
Mg2+ 40.5% residual activity at 5 mM [Candida] boidinii
Pyridine 19.8% residual activity at 5% (v/v) [Candida] boidinii
tetrahydrofuran 24.1% residual activity at 5% (v/v) [Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 t-butyl 6-cyano-(5R)-hydroxy-3-oxohexanoate at pH 7.0 and 25°C [Candida] boidinii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 115.1% activity at 5 mM [Candida] boidinii
K+ 114.4% activity at 5 mM [Candida] boidinii
Mn2+ 149.2% activity at 5 mM [Candida] boidinii
Na+ 128.4% activity at 5 mM [Candida] boidinii

Organism

Organism UniProt Comment Textmining
[Candida] boidinii
-
-
-
[Candida] boidinii CGMCC 9446
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, and Superdex 200 gel filtration [Candida] boidinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.48
-
 crude extract, at pH 7.0 and 25°C [Candida] boidinii
4.65
-
 after 9.7fold purification, at pH 7.0 and 25°C [Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
t-butyl 6-cyano-(5R)-hydroxy-3-oxohexanoate + NADPH + H+ high stereoselectivity, 94% conversion in 4 h [Candida] boidinii t-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate + NADP+
-
 ?
t-butyl 6-cyano-(5R)-hydroxy-3-oxohexanoate + NADPH + H+ high stereoselectivity, 94% conversion in 4 h [Candida] boidinii CGMCC 9446 t-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE [Candida] boidinii

Synonyms

Synonyms Comment Organism
crc1
-
 [Candida] boidinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 [Candida] boidinii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 45 the activity increases as temperature increases from 10 to 35°C. However, the activity decreases rapidly when temperature is above 35°C. The enzyme shows more than 60% activity between 30 and 45°C [Candida] boidinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 55 about 60% of enzymatic activity is maintained after 30 min of incubation at 35°C. However, the activity is completely lost after incubation at 55°C for 30 min [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 [Candida] boidinii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 the enzyme shows more than 70% activity between pH 6.5 and 8.0 [Candida] boidinii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7.5 the enzyme is highly stable in acidic environment and more than 80% of the activity is maintained when exposed to pH 5.5-7.5 for 60 min at 25°C [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on [Candida] boidinii