Crystallization (Comment) | Organism |
---|---|
computational model of docking of 4¢-methoxyacetophenone to the Q245H mutant | Sporidiobolus salmonicolor |
Protein Variants | Comment | Organism |
---|---|---|
Q245H | inversion of enantioselectivity from (R)- to (S)-configuration of reduced acetophenone product, with enhanced enantioselectivity | Sporidiobolus salmonicolor |
Q245L | inversion of enantioselectivity from (R)- to (S)-configuration of reduced acetophenone product, with enhanced enantioselectivity | Sporidiobolus salmonicolor |
Q245P | inversion of enantioselectivity from (R)- to (S)-configuration of reduced acetophenone product, with enhanced enantioselectivity | Sporidiobolus salmonicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sporidiobolus salmonicolor | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.009 | - |
substrate 4-tert-butylacetophenone, mutant Q245L | Sporidiobolus salmonicolor |
0.011 | - |
substrate 4-methylacetophenone, wild-type | Sporidiobolus salmonicolor |
0.011 | - |
substrate 4-tert-butylacetophenone, wild-type | Sporidiobolus salmonicolor |
0.013 | - |
substrate 4-bromoacetophenone, wild-type | Sporidiobolus salmonicolor |
0.014 | - |
substrate 4-fluoroacetophenone, wild-type | Sporidiobolus salmonicolor |
0.016 | - |
substrate 4-methoxyacetophenone, mutant Q245H | Sporidiobolus salmonicolor |
0.02 | - |
substrate 4-chloroacetophenone, wild-type | Sporidiobolus salmonicolor |
0.02 | - |
substrate 4-methoxyacetophenone, mutant Q245L | Sporidiobolus salmonicolor |
0.02 | - |
substrate 4-methoxyacetophenone, wild-type | Sporidiobolus salmonicolor |
0.02 | - |
substrate 4-methylacetophenone, mutant Q245L | Sporidiobolus salmonicolor |
0.025 | - |
substrate 4-fluoroacetophenone, mutant Q245P | Sporidiobolus salmonicolor |
0.025 | - |
substrate 4-methylacetophenone, mutant Q245H | Sporidiobolus salmonicolor |
0.028 | - |
substrate acetophenone, wild-type | Sporidiobolus salmonicolor |
0.032 | - |
substrate 4-tert-butylacetophenone, mutant Q245H | Sporidiobolus salmonicolor |
0.036 | - |
substrate 4-fluoroacetophenone, mutant Q245L | Sporidiobolus salmonicolor |
0.039 | - |
substrate acetophenone, mutant Q245P | Sporidiobolus salmonicolor |
0.045 | - |
substrate 4-methylacetophenone, mutant Q245P | Sporidiobolus salmonicolor |
0.047 | - |
substrate 4-bromoacetophenone, mutant Q245L | Sporidiobolus salmonicolor |
0.062 | - |
substrate 4-methoxyacetophenone, mutant Q245P | Sporidiobolus salmonicolor |
0.067 | - |
substrate 4-chloroacetophenone, mutant Q245L | Sporidiobolus salmonicolor |
0.072 | - |
substrate 4-fluoroacetophenone, mutant Q245H | Sporidiobolus salmonicolor |
0.084 | - |
substrate 4-tert-butylacetophenone, mutant Q245P | Sporidiobolus salmonicolor |
0.085 | - |
substrate acetophenone, mutant Q245H | Sporidiobolus salmonicolor |
0.086 | - |
substrate acetophenone, mutant Q245L | Sporidiobolus salmonicolor |
0.203 | - |
substrate 4-bromoacetophenone, mutant Q245H | Sporidiobolus salmonicolor |
0.238 | - |
substrate 4-chloroacetophenone, mutant Q245H | Sporidiobolus salmonicolor |
0.309 | - |
substrate 4-chloroacetophenone, mutant Q245P | Sporidiobolus salmonicolor |
0.403 | - |
substrate 4-bromoacetophenone, mutant Q245P | Sporidiobolus salmonicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 4-fluoroacetophenone + 2 NADPH + 2 H+ | - |
Sporidiobolus salmonicolor | (1S)-1-(4-fluorophenyl)ethanol + (1R)-1-(4-fluorophenyl)ethanol + 2 NADP+ | wild-type, (R)-configuration of product with 46% enantiomeric excess, mutant Q245H, (S)-configuration with 92%, mutant Q245P, with 90%, mutant Q245L, with 93% enantiomeric excess, respectively | ? | |
4-bromoacetophenone + NADPH + H+ | - |
Sporidiobolus salmonicolor | (1S)-1-(4-bromophenyl)ethanol + (1R)-1-(4-bromophenyl)ethanol + NADP+ | wild-type, (R)-configuration of product with 42% enantiomeric excess, mutant Q245H, (S)-configuration with 92%, mutant Q245P, with 98%, mutant Q245L, with 97% enantiomeric excess, respectively | ? | |
4-chloroacetophenone + NADPH + H+ | - |
Sporidiobolus salmonicolor | (1S)-1-(4-chlorophenyl)ethanol + (1R)-1-(4-chlorophenyl)ethanol + NADP+ | wild-type, (R)-configuration of product with 14% enantiomeric excess, mutant Q245H, (S)-configuration with 90%, mutant Q245P, with 96%, mutant Q245L, with 96% enantiomeric excess, respectively | ? | |
4-methoxyacetophenone + NADPH | - |
Sporidiobolus salmonicolor | (1S)-1-(4-methoxyphenyl)ethanol + (1R)-1-(4-methoxyphenyl)ethanol + NADP+ | wild-type, (R)-configuration of product with 57% enantiomeric excess, mutant Q245H, (S)-configuration with 79%, mutant Q245P, with 98%, mutant Q245L, with 96% enantiomeric excess, respectively | ? | |
4-methylacetophenone + NADPH + H+ | - |
Sporidiobolus salmonicolor | (1S)-1-(4-methylphenyl)ethanol + (1R)-1-(4-methylphenyl)ethanol + NADP+ | wild-type, (R)-configuration of product with 59% enantiomeric excess, mutant Q245H, (S)-configuration with 95%, mutant Q245P, with 96%, mutant Q245L, with 95% enantiomeric excess, respectively | ? | |
4-tert-butylacetophenone + NADPH + H+ | - |
Sporidiobolus salmonicolor | (1S)-1-(4-tert-butylphenyl)ethanol + (1R)-1-(4-tert-butylphenyl)ethanol + NADP+ | wild-type, (R)-configuration of product with 31% enantiomeric excess, mutant Q245H, (S)-configuration with 96%, mutant Q245P, with 99%, mutant Q245L, with 99% enantiomeric excess, respectively | ? | |
acetophenone + NADPH + H+ | - |
Sporidiobolus salmonicolor | (1S)-1-phenylethanol + (1R)-1-phenylethanol + NADP+ | wild-type, (R)-configuration of product with 42% enantiomeric excess, mutant Q245H, (S)-configuration with 78%, mutant Q245P, with 64%, mutant Q245L, with 82% enantiomeric excess, respectively | ? |