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Literature summary for 1.1.1.184 extracted from
- Purification and properties of an NADPH-dependent carbonyl reductase from human brain. Relationship to prostaglandin 9-ketoreductase and xenobiotic ketone reductase (1981), J. Biol. Chem., 256, 1206-1213.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Oxo-4H-benzopyran-2-carboxylic acid | - |
Homo sapiens |  |
| chlorogenic acid | - |
Homo sapiens | |
| chlorpromazine | - |
Homo sapiens | |
| Cibacron blue | 3G-A | Homo sapiens | |
| D-catechin | - |
Homo sapiens | |
| dicoumarol | - |
Homo sapiens | |
| Ethacrynic acid | - |
Homo sapiens | |
| FAD | - |
Homo sapiens | |
| flavonoids | - |
Homo sapiens | |
| indomethacin | - |
Homo sapiens | |
| iodoacetate | - |
Homo sapiens | |
| NADP+ | - |
Homo sapiens | |
| p-hydroxymercuribenzoate | - |
Homo sapiens | |
| quercetin | - |
Homo sapiens | |
| Retin | - |
Homo sapiens | |
| Tetramethyleneglutaric acid | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | overview | Homo sapiens | |
| 0.004 | 0.01 | NADPH | + 4-nitrobenzaldehyde, Km depending on enzyme form | Homo sapiens | |
| 0.015 | 0.017 | ubiquinone-1 | Km depending on enzyme form | Homo sapiens | |
| 0.045 | 0.06 | menadione | Km depending on enzyme form | Homo sapiens | |
| 0.13 | - |
daunorubicin | enzyme form CR8 | Homo sapiens | |
| 0.45 | - |
prostaglandin E1 | enzyme form CR8 | Homo sapiens | |
| 1.6 | 2 | 4-nitrobenzaldehyde | Km depending on enzyme form | Homo sapiens | |
| 4 | 5 | Phenylglyoxal | Km depending on enzyme form | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | detected in enzyme by atomic absorption spectroscopy | Homo sapiens | |
| Magnesium | detected in enzyme by atomic absorption spectroscopy | Homo sapiens | |
| Zinc | detected in enzyme by atomic absorption spectroscopy | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
gel filtration, SDS-PAGE, amino acid analysis | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3 molecular forms: CR7, CR8, CR8.5 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricyanide + NADPH | - |
Homo sapiens | 2 ferrocyanide + NADP+ + H+ | - |
? | |
| 2,3-bornanedione + NADPH | - |
Homo sapiens | ? | - |
? | |
| 4-carboxybenzaldehyde + NADPH | - |
Homo sapiens | 4-carboxybenzyl alcohol + NADP+ | - |
? | |
| 4-nitrobenzaldehyde + NADPH | - |
Homo sapiens | 4-nitrobenzyl alcohol + NADP+ | - |
? | |
| 9,10-phenanthrenequinone + NADPH | - |
Homo sapiens | ? | - |
? | |
| alpha-tocopherolquinone + NADPH | - |
Homo sapiens | ? | - |
? | |
| daunorubicin + NADPH | - |
Homo sapiens | ? | - |
? | |
| duroquinone + NADPH | - |
Homo sapiens | ? | - |
? | |
| hydrindantin + NADPH | - |
Homo sapiens | ? | - |
? | |
| indole 3-acetaldehyde + NADPH | - |
Homo sapiens | indole-3-ol + NADP+ | - |
? | |
| menadione + NADPH | - |
Homo sapiens | ? | - |
? | |
| methylglyoxal + NADPH | - |
Homo sapiens | ? | - |
? | |
| additional information | overview | Homo sapiens | ? | - |
? | |
| NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Homo sapiens | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
| phenylglyoxal + NADPH | - |
Homo sapiens | ? | - |
? | |
| prostaglandin A1-GSH + NADPH | - |
Homo sapiens | ? | - |
? | |
| prostaglandin E1 + NADPH + H+ | - |
Homo sapiens | ? | - |
r | |
| ubiquinone-1 + NADPH | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
menadione, 4-nitrobenzaldehyde, daunorubicin, in sodium phosphate buffer | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | pro-4S hydrogen atom of the nicotinamide ring of NADPH is transferred to the substrate | Homo sapiens | |
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