Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | adding sodium metabisulfite, ascorbic acid, dithiothreitol, and EDTA to the extraction buffer and using Tris-HCl (pH 7.5) or potassium phosphate (pH 7.5) fails to enhance enzyme activity | Persicaria minor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | isoform geraniol-DH I shows 72% residual activity and geraniol-DH II shows 75% residual activity at 1 mM | Persicaria minor | |
cyanide | isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM | Persicaria minor | |
iodoacetamide | isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM | Persicaria minor | |
MgCl2 | isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 45% residual activity at 5 mM | Persicaria minor | |
Sodium azide | isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 90% residual activity at 1 mM | Persicaria minor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
NADP+ | isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor | |
0.0588 | - |
NADP+ | isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor | |
0.185 | - |
geraniol | isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor | |
0.4 | - |
geraniol | isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor | |
0.435 | - |
cinnamyl alcohol | isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor | |
0.556 | - |
cinnamyl alcohol | isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor | |
0.714 | - |
nerol | isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor | |
0.741 | - |
linalool | isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor | |
1.212 | - |
nerol | isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor | |
1.333 | - |
linalool | isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor | |
2.222 | - |
3,7-dimethyloct-6-en-1-ol | isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor | |
10 | - |
3,7-dimethyloct-6-en-1-ol | isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE | Persicaria minor |
58000 | - |
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE | Persicaria minor |
60000 | - |
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE | Persicaria minor |
62000 | - |
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE | Persicaria minor |
100000 | - |
isoform geraniol-DH I, gel filtration | Persicaria minor |
120000 | - |
isoform geraniol-DH II, gel filtration | Persicaria minor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Persicaria minor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Toyopearl 650M column chromatography, phenyl Toyopearl 650 M column chromatography, and AF-Red Toyopearl 650ML column chromatography | Persicaria minor |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Persicaria minor | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
cell free extract, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor |
0.175 | - |
isoform geraniol-DH I, after 116.7fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor |
0.261 | - |
isoform geraniol-DH II, after 173.8fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C | Persicaria minor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-citronellol + NADP+ | compared to geraniol, isoform geraniol-DH I shows 72% activity and isoform geraniol-DH II shows 63% activity with this substrate | Persicaria minor | ? | - |
? | |
borneol + NADP+ | compared to geraniol, isoform geraniol-DH I shows 52% activity and isoform geraniol-DH II shows 29% activity with this substrate | Persicaria minor | ? | - |
? | |
carveol + NADP+ | compared to geraniol, isoform geraniol-DH I shows 44% activity with this substrate | Persicaria minor | ? | - |
? | |
cinnamyl alcohol + NADP+ | compared to geraniol, isoform geraniol-DH I shows 60% activity and isoform geraniol-DH II shows 50% activity with this substrate | Persicaria minor | ? | - |
? | |
geraniol + NADP+ | 100% activity | Persicaria minor | geranial + NADPH + H+ | - |
? | |
linalool + NADP+ | compared to geraniol, isoform geraniol-DH I shows 42% activity and isoform geraniol-DH II shows 92% activity with this substrate | Persicaria minor | ? | - |
? | |
additional information | tetrahydrogeraniol, tetrahydrolinalool, menthol, benzyl alcohol, and farnesol are no substrates for both isoforms. Isoform geraniol-DH II does not react with 3,7-dihydrolinalool, and isoform geraniol-DH I does not react with carveol | Persicaria minor | ? | - |
? | |
nerol + NADP+ | compared to geraniol, isoform geraniol-DH I shows 64% activity and isoform geraniol-DH II shows 50% activity with this substrate | Persicaria minor | ? | - |
? | |
p-cumic alcohol + NADP+ | compared to geraniol, isoform geraniol-DH I shows 56% activity and isoform geraniol-DH II shows 46% activity with this substrate | Persicaria minor | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE | Persicaria minor |
heterodimer | 1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE | Persicaria minor |
Synonyms | Comment | Organism |
---|---|---|
geraniol-DH I | isoform | Persicaria minor |
geraniol-DH II | isoform | Persicaria minor |
NADP+-dependent geraniol dehydrogenase | - |
Persicaria minor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
isoforms geraniol-DH I and geraniol-DH II | Persicaria minor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | rapid inactiviation of isoform geraniol-DH I occurs from 30-60°C with 60% activity at 40°C and 40% residual activity at 60°C. At 70°C the isoform shows less than 20% residual activity. Isoform geraniol-DH II retains over 80% activity after 10 min at 40°C, while from 30-50°C the activity decreases slightly and is lost at temperatures above 50°C | Persicaria minor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
isoform geraniol-DH I | Persicaria minor |
9.5 | - |
isoform geraniol-DH II | Persicaria minor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | isoforms geraniol-DH I and geraniol-DH II show less than 40% activity below pH 6.5. Isoform geraniol-DH II shows no activity at pH 10.0, while isoform geraniol-DH I shows about 50% activity. Both isoforms are inactive at pH 6.0 and below | Persicaria minor |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Persicaria minor | isoform geraniol-DH II, isoelectric focusing | - |
3.5 |
Persicaria minor | isoform geraniol-DH I, isoelectric focusing | - |
8.8 |