Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis | Carpoglyphus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
geraniol | pH 9.0, 25°C | Carpoglyphus lactis | |
0.0596 | - |
NAD+ | pH 9.0, 25°C | Carpoglyphus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42800 | - |
x * 42800, SDS-PAGE | Carpoglyphus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + NAD+ | Carpoglyphus lactis | the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone | geranial + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carpoglyphus lactis | B2NI94 | astigmatid mite | - |
Purification (Comment) | Organism |
---|---|
native enzyme 1879fold to homogeneity by ammonium sulfate fractionation, followed by hydrophobic interactin and adsorption chromatography, anion exchange chromatography, and gel filtration in seven purification steps, overview | Carpoglyphus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31 | - |
purified enzyme | Carpoglyphus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
E,E-farnesol + NAD+ | - |
Carpoglyphus lactis | ? | - |
? | |
geraniol + NAD+ | the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone | Carpoglyphus lactis | geranial + NADH + H+ | - |
? | |
geraniol + NAD+ | stereospecific reaction, no activity with nerol | Carpoglyphus lactis | geranial + NADH + H+ | - |
? | |
additional information | substrate specificity, poor activity with citronellol, 3-methyl-2-buten-1-ol, 1-octanol, 1-butanol, 2-buten-1-ol, and ethanol, overview | Carpoglyphus lactis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42800, SDS-PAGE | Carpoglyphus lactis |
Synonyms | Comment | Organism |
---|---|---|
GeDH | - |
Carpoglyphus lactis |
More | the enzyme is a member of the medium-chain dehydrogenase/reductase family | Carpoglyphus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Carpoglyphus lactis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
60 | temprature profile, overview | Carpoglyphus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Carpoglyphus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for, no activity with NADP+ | Carpoglyphus lactis |