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Literature summary for 1.1.1.183 extracted from

  • Potty, V.H.; Bruemmer, J.H.
    Oxidation of geraniol by enzyme system from orange (1970), Phytochemistry, 9, 1003-1007.
No PubMed abstract available
Search for more literature for 1.1.1.183

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol protects enzyme from inhibition Citrus sp.

Inhibitors

Inhibitors Comment Organism Structure
(+)-limonene 100% inhibition at 0.488 mM Citrus sp.
8-hydroxyquinoline 50% decrease at 0.67 mM Citrus sp.
alpha,alpha'-dipyridyl 50% inhibition at 25 mM Citrus sp.
alpha-terpineol about 55% inhibition at 0.56 mM Citrus sp.
EDTA 50% inhibition at 67.7 mM Citrus sp.
iodoacetamide
-
 Citrus sp.
linalool about 55% inhibition at 1.12 mM Citrus sp.
o-phenanthroline 50% inhibition at 16.7 mM Citrus sp.
oxidized glutathione reaction completely stopped at 3.333 mM Citrus sp.
Sodium azide 50% inhibition at 83 mM Citrus sp.
Zn2+ 50% inhibition 1.67 mM Citrus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0465
-
 geraniol pH 9.0 Citrus sp.
0.0833
-
 geraniol pH 7.0 Citrus sp.
0.454
-
 NADP+ pH 9.0 Citrus sp.
0.83
-
 NADP+ pH 7.0 Citrus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ suggested to be part of the enzyme Citrus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
 estimation, gel filtration Citrus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geraniol + NADP+ Citrus sp. key intermediate in terpenoid biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Citrus sp.
-
 orange
-

Purification (Commentary)

Purification (Comment) Organism
partially, ammonium sulfate fractionation, gel filtration, anion exchange chromatography Citrus sp.

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Citrus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 nerol shows 60% of the rate with geraniol, farnesol about 11% and citronellol 8% Citrus sp.
0.203
-
-
 Citrus sp.

Storage Stability

Storage Stability Organism
-19°C, dialyzed, rapid loss of activity Citrus sp.
-96°C, undialyzed ammonium sulfate fraction, 6 months, quite active Citrus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citronellol + NADP+
-
 Citrus sp. citronellal + NADPH
-
 ?
farnesol + NADP+
-
 Citrus sp. farnesal + NADPH
-
 ?
geraniol + NADP+
-
 Citrus sp. geranial + NADPH
-
 r
geraniol + NADP+ key intermediate in terpenoid biosynthesis Citrus sp. ?
-
 ?
additional information nerol, farnesol and citronellol are oxidized at slower rates Citrus sp. ?
-
 ?
nerol + NADP+
-
 Citrus sp. neral + NADPH + H+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 geranial reduction Citrus sp.
9
-
 geraniol oxidation Citrus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10 below 5 complete and irreversible inactivation after 5 min Citrus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 below pH 5 complete and irreversible inactivation after 5 min Citrus sp.

Cofactor

Cofactor Comment Organism Structure
additional information NAD+ is ineffective Citrus sp.
NADP+
-
 Citrus sp.
NADPH
-
 Citrus sp.