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Literature summary for 1.1.1.177 extracted from
- Structural and functional analysis of the gpsA gene product of Archaeoglobus fulgidus: A glycerol-3-phosphate dehydrogenase with an unusual NADP+ preference (2004), Protein Sci., 13, 3161-3171.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Archaeoglobus fulgidus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glyceraldehyde 3-phosphate | - |
Archaeoglobus fulgidus |  |
| L-alpha-glycerol 3-phosphate | - |
Archaeoglobus fulgidus | |
| NADP+ | - |
Archaeoglobus fulgidus | |
| NADPH | - |
Archaeoglobus fulgidus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
NADPH | pH 6.6, 70°C | Archaeoglobus fulgidus | |
| 0.1 | - |
L-alpha-glycerol 3-phosphate | pH 6.6, 70°C | Archaeoglobus fulgidus | |
| 0.8 | - |
NADP+ | pH 6.6, 70°C | Archaeoglobus fulgidus | |
| 1 | - |
D-glyceraldehyde 3-phosphate | pH 6.6, 70°C | Archaeoglobus fulgidus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
recombinant enzyme | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4 | - |
pH 6.6, 70°C, substrate L-alpha-glycerol 3-phosphate | Archaeoglobus fulgidus |
| 44 | - |
pH 6.6, 70°C, substrate D-glyceraldehyde 3-phosphate | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + NADPH | - |
Archaeoglobus fulgidus | L-alpha-glycerol 3-phosphate + NADP+ | - |
r | |
| L-alpha-glycerol 3-phosphate + NADP+ | - |
Archaeoglobus fulgidus | D-glyceraldehyde 3-phosphate + NADPH | - |
r | |
| additional information | no enzymic activity with dihydroxyacetone, glycerol, glycerol-2-phosphate, D-glyceraldehyde-3-phosphate, DL-glyceraldehyde, D-erythrose-4-phosphate, D-fructose-4-phosphate | Archaeoglobus fulgidus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | crystallization data | Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Archaeoglobus fulgidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
30 min, in presence of 200 mM potassium phosphate or 100 mM NaCl or 50 mM ammonium sulfate, completely stable | Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
or below, reduction reaction | Archaeoglobus fulgidus |
| 9.5 | - |
or above, oxidation reaction | Archaeoglobus fulgidus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Archaeoglobus fulgidus | |
| NADPH | - |
Archaeoglobus fulgidus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KI-value for NADPH is below 0.005 mM | Archaeoglobus fulgidus | |
| 0.005 | - |
NADP+ | pH 6.6, 70°C | Archaeoglobus fulgidus | |
| 0.1 | - |
D-glyceraldehyde 3-phosphate | pH 6.6, 70°C | Archaeoglobus fulgidus | |
| 17 | - |
L-alpha-glycerol 3-phosphate | pH 6.6, 70°C | Archaeoglobus fulgidus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Archaeoglobus fulgidus | deduced from gene sequence | - |
5 |
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