Literature summary for 1.1.1.176 extracted from

Harris, S.C.; Devendran, S.; Mendez-Garcia, C.; Mythen, S.M.; Wright, C.L.; Fields, C.J.; Hernandez, A.G.; Cann, I.; Hylemon, P.B.; Ridlon, J.M.
Bile acid oxidation by Eggerthella lenta strains C592 and DSM 2243T (2018), Gut Microbes, 9, 523-539 .

Search for more literature for 1.1.1.176

Inhibitors

Inhibitors	Comment	Organism	Structure
H2	H2 strongly inhibits bile acid oxidation by the enzyme	Eggerthella lenta

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09678	-	deoxycholic acid	with NAD+ as cosubstrate, pH and temperature not specified in the publication	Eggerthella lenta
0.19162	-	12-oxolithocholic acid	with NADH as cosubstrate, pH and temperature not specified in the publication	Eggerthella lenta

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
12-oxolithocholic acid + NADH + H+	Eggerthella lenta	-	deoxycholic acid + NAD+	-	r
12-oxolithocholic acid + NADH + H+	Eggerthella lenta C592	-	deoxycholic acid + NAD+	-	r
12-oxolithocholic acid + NADH + H+	Eggerthella lenta DSM 2243T	-	deoxycholic acid + NAD+	-	r
12-oxolithocholic acid + NADPH + H+	Eggerthella lenta	-	deoxycholic acid + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Eggerthella lenta	-	-	-
Eggerthella lenta C592	-	-	-
Eggerthella lenta DSM 2243T	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Talon metal affinity column chromatography	Eggerthella lenta

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
12-oxolithocholic acid + NADH + H+	-	Eggerthella lenta	deoxycholic acid + NAD+	-	r
12-oxolithocholic acid + NADH + H+	19.6% activity compared to deoxycholic acid	Eggerthella lenta	deoxycholic acid + NAD+	-	?
12-oxolithocholic acid + NADH + H+	-	Eggerthella lenta C592	deoxycholic acid + NAD+	-	r
12-oxolithocholic acid + NADH + H+	19.6% activity compared to deoxycholic acid	Eggerthella lenta C592	deoxycholic acid + NAD+	-	?
12-oxolithocholic acid + NADH + H+	-	Eggerthella lenta DSM 2243T	deoxycholic acid + NAD+	-	r
12-oxolithocholic acid + NADH + H+	19.6% activity compared to deoxycholic acid	Eggerthella lenta DSM 2243T	deoxycholic acid + NAD+	-	?
12-oxolithocholic acid + NADPH + H+	-	Eggerthella lenta	deoxycholic acid + NADP+	-	r
cholic acid + NAD+	98.5% activity compared to deoxycholic acid	Eggerthella lenta	3-oxocholic acid + NADH + H+	-	r
cholic acid + NAD+	98.5% activity compared to deoxycholic acid	Eggerthella lenta C592	3-oxocholic acid + NADH + H+	-	r
cholic acid + NAD+	98.5% activity compared to deoxycholic acid	Eggerthella lenta DSM 2243T	3-oxocholic acid + NADH + H+	-	r
deoxycholic acid + NAD+	100% activity	Eggerthella lenta	12-oxolithocholic acid + NADH + H+	-	r
deoxycholic acid + NADP+	3.14% activity compared to NAD+	Eggerthella lenta	12-oxolithocholic acid + NADH + H+	-	r
glycocholic acid + NAD+	81.08% activity compared to deoxycholic acid	Eggerthella lenta	3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH	-	r
glycocholic acid + NAD+	81.08% activity compared to deoxycholic acid	Eggerthella lenta C592	3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH	-	r
glycocholic acid + NAD+	81.08% activity compared to deoxycholic acid	Eggerthella lenta DSM 2243T	3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH	-	r
glycodeoxycholic acid + NAD+	97.8% activity compared to deoxycholic acid	Eggerthella lenta	? + NADH + H+	-	r
additional information	no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid	Eggerthella lenta	?	-	-
additional information	no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid	Eggerthella lenta C592	?	-	-
additional information	no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid	Eggerthella lenta DSM 2243T	?	-	-
taurocholic acid + NAD+	98.95% activity compared to deoxycholic acid	Eggerthella lenta	? + NADH + H+	-	r
taurodeoxycholic acid + NAD+	90.84% activity compared to deoxycholic acid	Eggerthella lenta	? + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
?	x * 27500, SDS-PAGE	Eggerthella lenta
?	x * 27078, calculated from amino acid sequence	Eggerthella lenta

Synonyms

Synonyms	Comment	Organism
12alpha-HSDH	-	Eggerthella lenta
Elen_2515	-	Eggerthella lenta

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.03	-	12-oxolithocholic acid	with NADH as cosubstrate, pH and temperature not specified in the publication	Eggerthella lenta
28.085	-	deoxycholic acid	with NAD+ as cosubstrate, pH and temperature not specified in the publication	Eggerthella lenta

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	7.5	pH optimum in the reductive direction with NAD+ as cofactor	Eggerthella lenta
9	-	pH optimum in the oxidative direction with NAD+ as cofactor	Eggerthella lenta

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	preferred cofactor	Eggerthella lenta
NADP+	-	Eggerthella lenta

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
10.59	-	12-oxolithocholic acid	with NADH as cosubstrate, pH and temperature not specified in the publication	Eggerthella lenta
290.19	-	deoxycholic acid	with NAD+ as cosubstrate, pH and temperature not specified in the publication	Eggerthella lenta