Application | Comment | Organism |
---|---|---|
degradation | new integrated chemo-enzymatic synthesis of ursodeoxycholic acid starting from sodium cholate by 7alpha- and 12alpha-hydroxysteroid dehydrogenases | Acinetobacter lwoffii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter lwoffii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially purified by ion-exchange chromatography | Acinetobacter lwoffii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.027 | - |
cell extract | Acinetobacter lwoffii |
0.3 | - |
purified enzyme | Acinetobacter lwoffii |
Storage Stability | Organism |
---|---|
4°C, 0.5 ml TEA-buffer, 3 days | Acinetobacter lwoffii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxycholic acid + NAD+ | - |
Acinetobacter lwoffii | 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
12alpha-HSDH | - |
Acinetobacter lwoffii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acinetobacter lwoffii |