KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
NAD+ | strain 89B | Pseudomonas sp. | |
0.39 | - |
NAD+ | strain 90A | Pseudomonas sp. | |
0.77 | - |
D-xylose | strain 90A | Pseudomonas sp. | |
2.9 | - |
D-xylose | strain 89B | Pseudomonas sp. | |
5.9 | - |
L-arabinose | strain 89B | Pseudomonas sp. | |
30 | - |
D-galactose | strain 89B | Pseudomonas sp. | |
100 | - |
L-arabinose | strain 90A | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + NAD+ | Pseudomonas sp. | low activity | ? + NADH | - |
? | |
D-glucose + NAD+ | Pseudomonas sp. | - |
? + NADH | - |
? | |
D-lyxose + NAD+ | Pseudomonas sp. | low activity | ? + NADH | - |
? | |
D-xylose + NAD+ | Pseudomonas sp. | - |
D-xylonolactone + NADH + H+ | - |
? | |
L-arabinose + NAD+ | Pseudomonas sp. | - |
? + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
strains 89B, 90A | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques, isoelectric focusing | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:D-xylose-grown cell | inducible enzyme synthesis by growth in D-xylose-containing medium | Pseudomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30.9 | - |
strain 89B | Pseudomonas sp. |
143 | - |
strain 90A | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + NAD+ | low activity | Pseudomonas sp. | ? + NADH | - |
? | |
D-glucose + NAD+ | - |
Pseudomonas sp. | ? + NADH | - |
? | |
D-lyxose + NAD+ | low activity | Pseudomonas sp. | ? + NADH | - |
? | |
D-xylose + NAD+ | - |
Pseudomonas sp. | D-xylonolactone + NADH + H+ | - |
? | |
L-arabinose + NAD+ | - |
Pseudomonas sp. | ? + NADH | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 55 | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.3 | 9 | stable within, strain 89B | Pseudomonas sp. |
6 | 7.5 | stable within, strain 90A | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. |