Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.17 extracted from

  • Krahulec, S.; Armao, G.C.; Bubner, P.; Klimacek, M.; Nidetzky, B.
    Polyol-specific long-chain dehydrogenases/reductases of mannitol metabolism in Aspergillus fumigatus: biochemical characterization and pH studies of mannitol 2-dehydrogenase and mannitol-1-phosphate 5-dehydrogenase (2009), Chem. Biol. Interact., 178, 274-282.
    View publication on PubMed
Search for more literature for 1.1.1.17

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Escherichia coli JM109 Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
K213A residual activity Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate + NADH + H+
-
 Aspergillus fumigatus D-mannitol 1-phosphate + NAD+
-
 r
D-mannitol 1-phosphate + NAD+
-
 Aspergillus fumigatus D-fructose 6-phosphate + NADH + H+
-
 r
additional information important role for Lys213 in the catalytic mechanism of M1PDH Aspergillus fumigatus ?
-
 ?

Synonyms

Synonyms Comment Organism
M1PDH
-
 Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0019
-
 D-mannitol 1-phosphate purified mutant K213A Aspergillus fumigatus

pH Range

pH Minimum pH Maximum Comment Organism
6 10 for the oxidative and reductive direction of the reactions under conditions where substrate is limiting (kcat/K) or saturating (kcat) Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Aspergillus fumigatus
NADH
-
 Aspergillus fumigatus