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Literature summary for 1.1.1.17 extracted from
- Mannitol-1-phosphate dehydrogenase of Escherichia coli (1986), Biochem. J., 239, 435-443.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | weak inhibition | Escherichia coli |  |
| 2,2'-bipyridyl | weak inhibition | Escherichia coli | |
| 4,7-phenanthroline | weak inhibition | Escherichia coli | |
| 5,5'-dithiobis-(2-nitrobenzoate) | complete inactivation at 0.24 mM | Escherichia coli | |
| diethyldithiocarbamate | 95% inactivation, prevented by presence of 2-mercaptoethanol | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Escherichia coli | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 1.14 Zn atoms/monomer | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22500 | - |
2 * 22500, SDS-PAGE | Escherichia coli |
| 45500 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol 1-phosphate + NAD+ | Escherichia coli | - |
D-fructose 6-phosphate + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Escherichia coli | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 502 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.03 M imidazole-HCl, pH 7.3, several months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol 1-phosphate + NAD+ | - |
Escherichia coli | D-fructose 6-phosphate + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 22500, SDS-PAGE | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
| NADH | - |
Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
1,10-phenanthroline | - |
Escherichia coli | |
| 11 | - |
2,2'-bipyridyl | - |
Escherichia coli | |
| 12 | - |
4,7-phenanthroline | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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