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Literature summary for 1.1.1.169 extracted from

  • Aikawa, Y.; Nishitani, Y.; Tomita, H.; Atomi, H.; Miki, K.
    Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP(.) (2016), Acta crystallogr. Sect. F, 72, 369-375.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene TK1968, recombinant coexpression of wild-type and mutant Tk-KPR enzyme dimers carrying a His6-tag and a Strep-tag on each monomer in Escherichia coli Thermococcus kodakarensis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant tagged enzyme mutant C84A, hanging drop vapour diffusion method, mixing of 0.001 ml of 10 mg /ml protein in 10 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, mM NADH, and 1 mM 2-oxopantoate, with 0.001 ml of reservoir solution containing 100 mM sodium acetate, pH 5.5, 10% w/v PEG 3350, 20% v/v 2-propanol, and equilibration against 0.5 ml reservoir solution, at 20°C, 1 week, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement method for modeling Thermococcus kodakarensis

Protein Variants

Protein Variants Comment Organism
C84A site-directed mutagenesis Thermococcus kodakarensis
W129A site-directed mutagenesis Thermococcus kodakarensis
Y60A site-directed mutagenesis Thermococcus kodakarensis

Inhibitors

Inhibitors Comment Organism Structure
CoA feedback inhibition Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-pantoate + NADP+ Thermococcus kodakarensis
-
2-dehydropantoate + NADPH + H+
-
r
(R)-pantoate + NADP+ Thermococcus kodakarensis ATCC BAA-918
-
2-dehydropantoate + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JGC2
-
-
Thermococcus kodakarensis ATCC BAA-918 Q5JGC2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant Tk-KPR enzyme dimers carrying a His6-tag and a Strep-tag on each monomer from Escherichia coli by affinity chromatography Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-pantoate + NADP+
-
Thermococcus kodakarensis 2-dehydropantoate + NADPH + H+
-
r
(R)-pantoate + NADP+
-
Thermococcus kodakarensis ATCC BAA-918 2-dehydropantoate + NADPH + H+
-
r

Subunits

Subunits Comment Organism
dimer crystal structure analysis Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
ketopantoate reductase
-
Thermococcus kodakarensis
KPR
-
Thermococcus kodakarensis
Tk-KPR
-
Thermococcus kodakarensis
TK1968
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
assay at Thermococcus kodakarensis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Thermococcus kodakarensis

General Information

General Information Comment Organism
physiological function ketopantoate reductase catalyzes the NAD(P)H-dependent reduction of 2-oxopantoate which is a step in the biosynthesis of coenzyme A Thermococcus kodakarensis