Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
prismic crystals, hanging drop vapor-diffusion technique | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Lys176 and Glu256 important for binding ketopantoate and the catalytic mechanism | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADPH | - |
Escherichia coli | |
0.02 | - |
NADPH | - |
Escherichia coli | |
0.06 | - |
ketopantoate | - |
Escherichia coli | |
0.12 | - |
ketopantoate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | - |
Stenotrophomonas maltophilia | |
additional information | does not require Mg2+, independent of divalent metal ions | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30500 | - |
3-5 * 30500 or 30000, SDS-PAGE | Stenotrophomonas maltophilia |
31100 | - |
1 * 31100, HPLC gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
33000 | - |
SDS-PAGE | Escherichia coli |
34000 | - |
1 * 34000 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ketopantoate + NADPH | Salmonella enterica subsp. enterica serovar Typhimurium | - |
pantoate + NADP+ | - |
? | |
ketopantoate + NADPH | Escherichia coli | pantothenate biosynthetic pathway | pantoate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9J4 | expressed in BL21(DE3) | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Stenotrophomonas maltophilia | - |
845 | - |
Stenotrophomonas maltophilia 845 | - |
845 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | SeMet substitution | Escherichia coli |
Purification (Comment) | Organism |
---|---|
FPLC, anion exchange chromatography, gel filtration | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
additional information | - |
3.8-fold higher specific activity with NADPH than with NADH | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ketopantoate + NADPH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pantoate + NADP+ | - |
? | |
ketopantoate + NADPH | pantothenate biosynthetic pathway | Escherichia coli | pantoate + NADP+ | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | 3.8-fold higher specific activity with NADPH than with NADH | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31100, HPLC gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
monomer | 1 * 34000 | Escherichia coli |
monomer | 1 * 33000, gel filtration | Escherichia coli |
oligomer | 3-5 * 30500 or 30000, SDS-PAGE | Stenotrophomonas maltophilia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
ketopantoate | - |
Escherichia coli | |
40 | - |
ketopantoate | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
NADPH | - |
Stenotrophomonas maltophilia | |
NADPH | - |
Escherichia coli |