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Literature summary for 1.1.1.169 extracted from

  • Matak-Vinkovic, D.; Vinkovic, M.; Saldanha, S.A.; Ashurst, J.L.; von Delft, F.; Inoue, T.; Miguel, R.N.; Smith, A.G.; Blundell, T.L.; Abell, C.
    Crystal structure of Escherichia coli ketopantoate reductase at 1.7 A resolution and insight into the enzyme mechanism (2001), Biochemistry, 40, 14493-14500.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
prismic crystals, hanging drop vapor-diffusion technique Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information Lys176 and Glu256 important for binding ketopantoate and the catalytic mechanism Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
NADPH
-
Escherichia coli
0.02
-
NADPH
-
Escherichia coli
0.06
-
ketopantoate
-
Escherichia coli
0.12
-
ketopantoate
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
additional information
-
Stenotrophomonas maltophilia
additional information does not require Mg2+, independent of divalent metal ions Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30500
-
3-5 * 30500 or 30000, SDS-PAGE Stenotrophomonas maltophilia
31100
-
1 * 31100, HPLC gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
33000
-
SDS-PAGE Escherichia coli
34000
-
1 * 34000 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ketopantoate + NADPH Salmonella enterica subsp. enterica serovar Typhimurium
-
pantoate + NADP+
-
?
ketopantoate + NADPH Escherichia coli pantothenate biosynthetic pathway pantoate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A9J4 expressed in BL21(DE3)
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Stenotrophomonas maltophilia
-
845
-
Stenotrophomonas maltophilia 845
-
845
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification SeMet substitution Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
FPLC, anion exchange chromatography, gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli
additional information
-
3.8-fold higher specific activity with NADPH than with NADH Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ketopantoate + NADPH
-
Salmonella enterica subsp. enterica serovar Typhimurium pantoate + NADP+
-
?
ketopantoate + NADPH pantothenate biosynthetic pathway Escherichia coli pantoate + NADP+
-
?
additional information
-
Escherichia coli ?
-
?
additional information 3.8-fold higher specific activity with NADPH than with NADH Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 31100, HPLC gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
monomer 1 * 34000 Escherichia coli
monomer 1 * 33000, gel filtration Escherichia coli
oligomer 3-5 * 30500 or 30000, SDS-PAGE Stenotrophomonas maltophilia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8
-
ketopantoate
-
Escherichia coli
40
-
ketopantoate
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Salmonella enterica subsp. enterica serovar Typhimurium
NADPH
-
Stenotrophomonas maltophilia
NADPH
-
Escherichia coli