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Literature summary for 1.1.1.169 extracted from

  • King, H.L.; Wilken, D.R.
    Separation and preliminary studies on 2-ketopantoyl lactone and 2-ketopantoic acid reductases of yeast (1972), J. Biol. Chem., 247, 4096-4098.
No PubMed abstract available
Search for more literature for 1.1.1.169

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 NRRL Y-2034
-
Saccharomyces cerevisiae NRRL Y-2034
-
 NRRL Y-2034
-

Purification (Commentary)

Purification (Comment) Organism
gel chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity with ketopantoic acid is 100%, with ketopantoyl lactone 6%, with oxoalacetic acid 4%, no activity with 2-ketoisovaleric acid, pyruvic acid, 3-hydroxypyruvic acid, 3-phosphohydroxypyruvic acid, 2-ketobutyric acid, 2-ketoglutaric acid, and acetaldehyde Saccharomyces cerevisiae
0.38
-
 at pH 6.5 using NADPH and ketopantoic acid Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ketopantoic acid + NADPH
-
 Saccharomyces cerevisiae pantoic acid + NADP+
-
 ?
ketopantoic acid + NADPH
-
 Saccharomyces cerevisiae NRRL Y-2034 pantoic acid + NADP+
-
 ?
additional information activity with ketopantoic acid is 100%, with ketopantoyl lactone 6%, with oxoalacetic acid 4%, no activity with 2-ketoisovaleric acid, pyruvic acid, 3-hydroxypyruvic acid, 3-phosphohydroxypyruvic acid, 2-ketobutyric acid, 2-ketoglutaric acid, and acetaldehyde Saccharomyces cerevisiae ?
-
 ?
additional information activity with ketopantoic acid is 100%, with ketopantoyl lactone 6%, with oxoalacetic acid 4%, no activity with 2-ketoisovaleric acid, pyruvic acid, 3-hydroxypyruvic acid, 3-phosphohydroxypyruvic acid, 2-ketobutyric acid, 2-ketoglutaric acid, and acetaldehyde Saccharomyces cerevisiae NRRL Y-2034 ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 reduction of ketopantoic acid with NADPH Saccharomyces cerevisiae
5.5
-
 reduction of ketopantoic acid with NADH Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
4.3 6.5 pH 4.3: about 10% of activity maximum, pH 6.5: about 40% of activity maximum Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADH less active than NADPH at 0.1 mM Saccharomyces cerevisiae
NADPH
-
 Saccharomyces cerevisiae