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Literature summary for 1.1.1.169 extracted from

  • Shimizu, S.; Kataoka, M.; Ching-Ming Chung, M.; Yamada, H.
    Ketopantoic acid reductase of Pseudomonas maltophilia 845. Purification, characterization, and role in pantothenate biosynthesis (1988), J. Biol. Chem., 263, 12077-12084.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
fine needle, at about 40% ammonium sulfate saturation Stenotrophomonas maltophilia

Inhibitors

Inhibitors Comment Organism Structure
Barbital 9.0% at 1 mM Stenotrophomonas maltophilia
D-pantoic acid noncompetitive with ketopantoic acid at saturation level of NADPH, noncompetitive with NADPH at unsaturation level of ketopantoic acid Stenotrophomonas maltophilia
Diphenylhydantoin 22.6% at 1 mM Stenotrophomonas maltophilia
iodoacetate sulfhydryl reagents, slightly inhibitory Stenotrophomonas maltophilia
NaAsO2 carbonyl reagent Stenotrophomonas maltophilia
NADP+ competitive with NADPH at unsaturation level of ketopantoic acid Stenotrophomonas maltophilia
p-chloromercuribenzoate sulfhydryl reagents, slightly inhibitory Stenotrophomonas maltophilia
phenylhydrazine carbonyl reagent Stenotrophomonas maltophilia
quercetin 12.0% at 0.1 mM Stenotrophomonas maltophilia
Semicarbazide carbonyl reagent Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0318
-
NADPH pH 7.0 Stenotrophomonas maltophilia
0.4
-
ketopantoic acid pH 7.0 Stenotrophomonas maltophilia
8.55
-
2-Keto-3-hydroxyisovalerate
-
Stenotrophomonas maltophilia
52.1
-
D-pantoic acid pH 8.0 Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ independent Stenotrophomonas maltophilia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30500
-
3-5 * 30500 or 30000, SDS-PAGE Stenotrophomonas maltophilia
87000
-
+/-5000, sedimentation equilibrium method Stenotrophomonas maltophilia
115000
-
+/-5000, gel filtration Stenotrophomonas maltophilia
140000 160000 +/-5000, HPLC gel filtration Stenotrophomonas maltophilia

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Stenotrophomonas maltophilia
-
845
-
Stenotrophomonas maltophilia 845
-
845
-

Purification (Commentary)

Purification (Comment) Organism
gel chromatography Stenotrophomonas maltophilia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
633
-
crystalline enzyme Stenotrophomonas maltophilia
669
-
-
Stenotrophomonas maltophilia

Storage Stability

Storage Stability Organism
4°C, 0.01 M Tris/HCl buffer, pH 7.4, 0.1 mM DTT, 0.2 M NaCl, 6 months Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-keto-3-hydroxyisovalerate + NADPH
-
Stenotrophomonas maltophilia ? + NADP+
-
r
2-keto-3-hydroxyisovalerate + NADPH
-
Stenotrophomonas maltophilia 845 ? + NADP+
-
r
ketopantoic acid + NADPH
-
Stenotrophomonas maltophilia D-pantoic acid + NADP+
-
r
ketopantoic acid + NADPH
-
Stenotrophomonas maltophilia 845 D-pantoic acid + NADP+
-
r
additional information high specificity, among a variety of carbonyl compounds only ketopantoic acid and 2-keto-3-hydroxyisovalerate can serve as substrate Stenotrophomonas maltophilia ?
-
?
additional information high specificity, among a variety of carbonyl compounds only ketopantoic acid and 2-keto-3-hydroxyisovalerate can serve as substrate Stenotrophomonas maltophilia 845 ?
-
?

Subunits

Subunits Comment Organism
oligomer 3-5 * 30500 or 30000, SDS-PAGE Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
reduction of ketopantoic acid Stenotrophomonas maltophilia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 60 15°C: about 70% of activity maximum, 60°C: about 85% of activity maximum Stenotrophomonas maltophilia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
30 min, pH 6-10 Stenotrophomonas maltophilia
60
-
10 min, stable below 60°C Stenotrophomonas maltophilia
70
-
10 min, 70% loss of activity Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reduction of ketopantoic acid Stenotrophomonas maltophilia
8.5
-
oxidation of D-pantoic acid Stenotrophomonas maltophilia

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 30°C, 30 min Stenotrophomonas maltophilia
7
-
stable below 60°C for 10 min Stenotrophomonas maltophilia

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADH Stenotrophomonas maltophilia
NADP+
-
Stenotrophomonas maltophilia
NADPH
-
Saccharomyces cerevisiae
NADPH specific Stenotrophomonas maltophilia