Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Chlorobaculum tepidum |
Protein Variants | Comment | Organism |
---|---|---|
F99A | the mutant shows 3.9fold higher Km and lower Vmax (8.95%) than the wild type enzyme | Chlorobaculum tepidum |
W196A | the mutant shows 8.7fold higher Km and lower Vmax (5.64%) than the wild type enzyme | Chlorobaculum tepidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00018 | - |
sepiapterin | wild type enzyme | Chlorobaculum tepidum | |
0.00072 | - |
sepiapterin | mutant enzyme F99A | Chlorobaculum tepidum | |
0.0016 | - |
sepiapterin | mutant enzyme W196A | Chlorobaculum tepidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobaculum tepidum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA bead chromatography, MonoQ column chromatography, and Superdex 200 gel filtration | Chlorobaculum tepidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+ | - |
Chlorobaculum tepidum | L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+ | - |
? | |
sepiapterin + NADPH + H+ | - |
Chlorobaculum tepidum | 7,8-dihydrobiopterin + NADP+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
72 | - |
the melting temperature of the wild type enzyme is at 72.77°C | Chlorobaculum tepidum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Chlorobaculum tepidum |