Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41 (DE3) | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Flufenamic acid | poor inhibition for 20alpha-hydroxyprogesterone oxidation | Rattus norvegicus | |
Meclofenamic acid | 20alpha-hydroxyprogesterone oxidation | Rattus norvegicus | |
additional information | not inhibited by indomethacin, tolmetin and steroidal anti-inflammatory drugs | Rattus norvegicus | |
synthetic non-steroidal oestrogens | hexoestrol most potent competitive inhibitor | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00195 | - |
9,10-phenanthrenequinone | +/-0.0004 | Rattus norvegicus | |
0.008 | - |
NADP+ | +/-0.0011 | Rattus norvegicus | |
0.009 | - |
20alpha-hydroxyprogesterone | +/-0.0001 | Rattus norvegicus | |
0.011 | - |
progesterone | +/-0.0017 | Rattus norvegicus | |
0.012 | - |
3alpha-hydroxy-5alpha-pregnan-20-one | +/-0.0035 | Rattus norvegicus | |
0.013 | - |
20alpha-hydroxy-5alpha-pregnan-3-one | +/-0.0035 | Rattus norvegicus | |
0.014 | - |
NADPH | +/-0.004 | Rattus norvegicus | |
0.0154 | - |
17alpha-hydroxyprogesterone | +/-0.0029 | Rattus norvegicus | |
0.3811 | - |
4-nitrobenzaldehyde | +/-0.0605 | Rattus norvegicus | |
120 | - |
D,L-glyceraldehyde | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
female, Sprague-Dawley, 2 weeks | - |
Purification (Comment) | Organism |
---|---|
anion exchange and affinity chromatography | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ | bibi mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ovary | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
reduction of 0.06 mM prostaglandin E2 | Rattus norvegicus |
0.007 | - |
reduction of 0.03 mM 17alpha-hydroxy-pregnenolone | Rattus norvegicus |
0.013 | - |
reduction of 100 mM D-glucose | Rattus norvegicus |
0.016 | - |
reduction of 0.03 mM prednisolone | Rattus norvegicus |
0.017 | - |
reduction of 100 mM D-galactose | Rattus norvegicus |
0.025 | - |
reduction of 0.03 mM prednisone | Rattus norvegicus |
0.067 | - |
reduction of 0.05 mM pregnenolone | Rattus norvegicus |
0.9 | - |
reduction of 0.06 mM 5alpha-pregnane-3,20-dione | Rattus norvegicus |
2.09 | - |
20alpha-hydroxyprogesterone | Rattus norvegicus |
2.5 | - |
oxidation of 0.06 mM 20alpha-hydroxy-5beta-pregnan-3-one | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, 2.8 mg/ml protein concentration, 20 mM potassium phosphate buffer, pH 7.0, 1 mM EDTA, 1 mM beta-mercaptoethanol, 30%, v/v, glycerol | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxypregnenolone + NADPH | - |
Rattus norvegicus | ? + NADP+ | - |
? | |
17alpha-hydroxyprogesterone + NADPH + H+ | - |
Rattus norvegicus | 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NADP+ | - |
? | |
20alpha-hydroxy-5alpha-pregnan-3-one + NADPH + H+ | - |
Rattus norvegicus | 5alpha-pregnan-20alpha,3alpha-diol + NADP+ | - |
r | |
3alpha-hydroxy-5-alpha-pregnan-20-one + NADPH + H+ | - |
Rattus norvegicus | 5alpha-pregnan-3alpha,20alpha-diol + NADP+ | - |
r | |
3alpha-hydroxy-5alpha-pregnan-20-one + NADPH + H+ | - |
Rattus norvegicus | 5alpha-pregnan-3alpha,20alpha-diol + NADP+ | - |
r | |
4-nitrobenzaldehyde + NADPH | - |
Rattus norvegicus | 4-nitrobenzyl alcohol + NADP+ | - |
? | |
9,10-phenanthrenequinone + NADPH | - |
Rattus norvegicus | NADP+ + ? | - |
? | |
DL-glyceraldehyde + NADPH | - |
Rattus norvegicus | glycerol + NADP+ | - |
? | |
additional information | high activity for neuroactive steroids, 9,10-phenanthrenequinone and 4-nitrobenzaldehyde, low activity for 17alpha hydroxypregnenolone, D,L-glyceraldehyde and prostaglandin E2, no activity for 20beta-hydroxy-pregn-4-en-3-one, andro-, testosterone, 17alpha-, 17beta-oestradiol, oestrone and 5alpha-dihydrotestosterone, other substrates: DL-glyceraldehyde, prostaglandin E2, D-glucose, prednisolone, D-galactose, prednisone, pregnenolone, 5alpha-pregnane-3,20-dione, 9,10-phenanthrenequinone | Rattus norvegicus | ? | - |
? | |
progesterone + NADPH | - |
Rattus norvegicus | 20alpha-hydroxyprogesterone + NADP+ | - |
r | |
prostaglandin E2 + NADPH | low activity | Rattus norvegicus | NADP+ + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.178 | - |
17alpha-hydroxyprogesterone | +/-0.8 | Rattus norvegicus | |
0.208 | - |
3alpha-hydroxy-5-alpha-pregnan-20-one | +/-1.6 | Rattus norvegicus | |
0.215 | - |
D,L-glyceraldehyde | +/-0.3 | Rattus norvegicus | |
0.785 | - |
20alpha-hydroxy-5alpha-pregnan-3-one | +/-4.3 | Rattus norvegicus | |
1.17 | - |
20alpha-hydroxyprogesterone | +/-0.2 | Rattus norvegicus | |
1.43 | - |
NADPH | +/-1.3 | Rattus norvegicus | |
1.52 | - |
NADP+ | +/-6.1 | Rattus norvegicus | |
1.65 | - |
progesterone | +/-6.7 | Rattus norvegicus | |
5.91 | - |
4-nitrobenzaldehyde | +/-33.4 | Rattus norvegicus | |
7.7 | - |
9,10-phenanthrenequinone | +/-26.9 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | strictly specific | Rattus norvegicus | |
NADPH | strictly specific | Rattus norvegicus |