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Literature summary for 1.1.1.149 extracted from

  • Pongsawasdi, P.; Anderson, B.M.
    Kinetic studies of rat ovarian 20alpha-hydroxysteroid dehydrogenase (1984), Biochim. Biophys. Acta, 799, 51-58.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Rattus norvegicus

General Stability

General Stability Organism
NADP+ protects against thermal inactivation, 20alpha-hydroxyprogesterone does not Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
17beta-estradiol 20alpha-hydroxyprogesterone analog, competitive or noncompetitive depending on variable substrate Rattus norvegicus
2'-phosphoadenosine diphosphoribose NADP+ analog, competitive or noncompetitive depending on variable substrate Rattus norvegicus
3-Aminopyridine adenine dinucleotide phosphate
-
Rattus norvegicus
additional information no inhibition by NADPH with saturating concentration of NADP+ Rattus norvegicus
NADPH competitive and noncompetitive product inhibition with respect to NADP+ depending on substrate concentrations Rattus norvegicus
progesterone noncompetitive product inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
NADP+ spectrophotometric, pH 6.8 Rattus norvegicus
0.00088
-
NADP+ fluorometric, pH 7.8 Rattus norvegicus
0.00102
-
20alpha-hydroxy-4-pregnen-3-one spectrophotometric, pH 6.8 Rattus norvegicus
0.00138
-
NADPH spectrophotometric, pH 6.8 Rattus norvegicus
0.00162
-
20alpha-hydroxy-4-pregnen-3-one fluorometric, pH 7.8 Rattus norvegicus
0.00186
-
thionicotinamide adenine dinucleotide phosphate NADP analog Rattus norvegicus
0.00208
-
3-acetylpyridine adenine dinucleotide phosphate NADP analog Rattus norvegicus
0.00538
-
progesterone spectrophotometric pH 6.8 Rattus norvegicus
0.0588
-
nicotinamide hypoxanthine dinucleotide phosphate NADP analog Rattus norvegicus
0.1429
-
nicotinamide 1,N6-ethenoadenine dinucleotide phosphate NADP analog Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration Rattus norvegicus
38000
-
1 * 38000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
female, nonpregnant, DUB:(SD)
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, affinity chromatography Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ bibi mechanism Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
ovary
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.48
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-15°C, 0.005 mg/ml, 0.01 M potassium phosphate buffer, pH 8.0, 50% propylene glycol, 0.2 M KCl, 2 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
20alpha-hydroxypregn-4-en-3-one + 3-acetylpyridine adenine dinucleotide phosphate
-
Rattus norvegicus ?
-
r
20alpha-hydroxypregn-4-en-3-one + nicotinamide 1,N6-ethenoadenine dinucleotide phosphate
-
Rattus norvegicus ?
-
r
20alpha-hydroxypregn-4-en-3-one + nicotinamide hypoxanthine dinucleotide phosphate
-
Rattus norvegicus ?
-
r
20alpha-hydroxypregn-4-en-3-one + thionicotinamide adenine dinucleotide phosphate
-
Rattus norvegicus ?
-
r
additional information no activity with 20alpha-hydroxy-4-pregnen-3-one Rattus norvegicus ?
-
?
progesterone + NADPH
-
Rattus norvegicus 20alpha-hydroxyprogesterone + NADP+
-
r

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
NADP+ protects against thermal inactivation Rattus norvegicus
25
-
20 min Rattus norvegicus
38
-
half-life 7.6 min Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
varying NADP+ or 20alpha-hydroxyprogesterone concentrations Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
3-acetylpyridine adenine dinucleotide phosphate coenzyme analog Rattus norvegicus
NADP+ coenzyme Rattus norvegicus
NADPH
-
Rattus norvegicus
nicotinamide 1,N6-ethenoadenine dinucleotide phosphate coenzyme analog Rattus norvegicus
nicotinamide hypoxanthine dinucleotide phosphate coenzyme analog Rattus norvegicus
thio-NADP+ coenzyme analog Rattus norvegicus