Literature summary for 1.1.1.148 extracted from

Hasnain, S.; Williamson, D.G.
Properties of the multiple forms of the soluble 17alpha-hydroxy steroid dehydrogenase of rabbit liver (1977), Biochem. J., 161, 279-283.

Search for more literature for 1.1.1.148

Inhibitors

Inhibitors	Comment	Organism	Structure
Epitestosterone	substrate inhibition for all isoforms at concentration above 0.003 mM, not observed with other substrates	Oryctolagus cuniculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0002	0.0052	Epitestosterone	for six isoforms	Oryctolagus cuniculus
0.001	0.0051	17alpha-estradiol 3-glucuronide	for six isoforms	Oryctolagus cuniculus
0.0024	0.0088	17alpha-estradiol	for six isoforms	Oryctolagus cuniculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39600	-	x * 39600, SDS-PAGE, all 8 forms	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	eight isoforms	-

Purification (Commentary)

Purification (Comment)	Organism
8 forms, six of them purified to homogenity: IA, IB, IC, IIC, IID, III	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Oryctolagus cuniculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.003	0.009	isoform IA, IB, IID, III, 17alpha-estradiol 3-glucuronide	Oryctolagus cuniculus
0.007	0.015	six isoforms, 17alpha-estradiol	Oryctolagus cuniculus
0.041	0.2	six isoforms, epitestosterone	Oryctolagus cuniculus
0.047	-	isoform IIC, 17alpha-estradiol 3-glucuronide	Oryctolagus cuniculus
0.18	-	isoform IC, 17alpha-estradiol 3-glucuronide	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
17alpha-estradiol + NAD(P)+	-	Oryctolagus cuniculus	estrone + NAD(P)H	-	?
17alpha-hydroxy-4-androsten-3-one + NADP+	epitestosterone	Oryctolagus cuniculus	4-androsten-3,17-dione + NADPH	-	r
estradiol-17alpha 3-galacturonide + NADP+	-	Oryctolagus cuniculus	estrone 3-galacturonide + NADPH	-	?
estradiol-17alpha 3-glucoside + NADP+	-	Oryctolagus cuniculus	estrone 3-glucoside + NADPH	-	?
estradiol-17alpha 3-glucuronide + NADP+	-	Oryctolagus cuniculus	estrone 3-glucuronide + NADPH	-	?

Subunits

Subunits	Comment	Organism
?	x * 39600, SDS-PAGE, all 8 forms	Oryctolagus cuniculus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Oryctolagus cuniculus
NADP+	-	Oryctolagus cuniculus

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Release 2023.1 (January 2023)