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Literature summary for 1.1.1.146 extracted from
- Conformational flexibility in crystal structures of human 11beta-hydroxysteroid dehydrogenase type I provide insights into glucocorticoid interconversion and enzyme regulation (2005), J. Biol. Chem., 280, 4639-4648.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning from clone 5193867, ATCC clone 7277078, expression of isozyme 11beta-HSD1 catalytic domain mutant C272S, comprising residues 24-292 proceeded by a 17-amino acid-affinity tag, in Escherichia coli strain DH10b-Tir | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant isozyme 11beta-HSD1 catalytic domain mutant C272S, the nanovolume crystallization method, the reservoir solution contains 20% PEG 3350, and 0.1 M MES, pH 6.2, soaking of crystals in 1 mM lutetium acetate reservoir solution, two crystal forms, X-ray diffraction structure determination and analysis at 1.55-2.45 A resolution | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | oxidation of the enzymes disulfides with K3Fe(CN)6 reduces the activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0096 | - |
cortisol | pH 7.5, 22°C, recombinant wild-type isozyme 11beta-HSD1 | Homo sapiens |  |
| 0.042 | - |
cortisol | pH 7.5, 22°C, recombinant wild-type isozyme 11beta-HSD1 oxidized with K3Fe(CN)6 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant isozyme 11beta-HSD1 catalytic domain mutant C272S from Escherichia coli strain DH10b-Tir by affinity chromatography | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cortisol + NADP+ | isozyme 11beta-HSD1, steroid binding structure, overview | Homo sapiens | cortisone + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | subunit structure and assembly, quarternary structure with interface-closed or interface-opened conformations, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 11beta-HSD1 | - |
Homo sapiens |
| 11beta-hydroxysteroid dehydrogenase type 1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.042 | - |
cortisol | pH 7.5, 22°C, recombinant wild-type isozyme 11beta-HSD1 | Homo sapiens | |
| 0.078 | - |
cortisol | pH 7.5, 22°C, recombinant wild-type isozyme 11beta-HSD1 oxidized with K3Fe(CN)6 | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Homo sapiens | |
| NADPH | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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