Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.146 extracted from

  • Schweizer, R.A.; Zurcher, M.; Balazs, Z.; Dick, B.; Odermatt, A.
    Rapid hepatic metabolism of 7-ketocholesterol by 11beta-hydroxysteroid dehydrogenase type 1: species-specific differences between the rat, human, and hamster enzyme (2004), J. Biol. Chem., 279, 18415-18424.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293 cells Homo sapiens
expression in HEK-293 cells Rattus norvegicus
expression in HEK-293 cells Mesocricetus auratus

Inhibitors

Inhibitors Comment Organism Structure
2'-hydroxyflavanone 0.2 mM, more than 90% inhibition of 7-oxocholesterol reduction in liver homogenates Mesocricetus auratus
2'-hydroxyflavanone 0.2 mM, more than 90% inhibition of 7-oxocholesterol reduction in liver homogenates Rattus norvegicus
carbenoxolone 0.01 mM, more than 90% inhibition of 7-oxocholesterol reduction in liver homogenates Mesocricetus auratus
carbenoxolone 0.01 mM, more than 90% inhibition of 7-oxocholesterol reduction in liver homogenates Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
corticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Homo sapiens
0.00031
-
11-Dehydrocorticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Homo sapiens
0.00031
-
11-Dehydrocorticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Rattus norvegicus
0.00038
-
7-oxocholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Mesocricetus auratus
0.0004
-
11-Dehydrocorticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Mesocricetus auratus
0.00042
-
7-oxocholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Rattus norvegicus
0.00049
-
7-oxocholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Homo sapiens
0.00049
-
7alpha-hydroxycholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Mesocricetus auratus
0.00073
-
7beta-hydroxycholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Mesocricetus auratus
0.00074
-
7beta-hydroxycholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Homo sapiens
0.00075
-
7beta-hydroxycholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Rattus norvegicus
0.0012
-
corticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Rattus norvegicus
0.0032
-
corticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Mesocricetus auratus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7beta-hydroxycholesterol + NADP+ Homo sapiens
-
7-oxocholesterol + NADPH
-
r
7beta-hydroxycholesterol + NADP+ Rattus norvegicus
-
7-oxocholesterol + NADPH
-
r
7beta-hydroxycholesterol + NADP+ Mesocricetus auratus
-
7-oxocholesterol + NADPH
-
r
corticosterone + NADP+ Homo sapiens
-
11-dehydrocorticosterone + NADPH
-
r
corticosterone + NADP+ Rattus norvegicus
-
11-dehydrocorticosterone + NADPH
-
r
corticosterone + NADP+ Mesocricetus auratus
-
11-dehydrocorticosterone + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mesocricetus auratus Q6R0J2
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus norvegicus
-
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
liver
-
Mesocricetus auratus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 7-oxocholesterol + 2 NADPH + 2 H+
-
Mesocricetus auratus 7beta-hydroxycholesterol + 7alpha-hydroxycholesterol + 2 NADP+ 40% and 60%, respectively, in lysates of HEK-293 cells and intact HEK-293 cells expressing 11beta-HSD1 r
7alpha-hydroxycholesterol + NADP+ in lysates of HEK-293 cells expressing 11beta-HSD1, no oxidation in intact HEK293 cells expressing 11beta-HSD1 Mesocricetus auratus 7-oxocholesterol + NADPH
-
r
7beta-hydroxycholesterol + NADP+
-
Homo sapiens 7-oxocholesterol + NADPH
-
r
7beta-hydroxycholesterol + NADP+
-
Rattus norvegicus 7-oxocholesterol + NADPH
-
r
7beta-hydroxycholesterol + NADP+
-
Mesocricetus auratus 7-oxocholesterol + NADPH
-
r
7beta-hydroxycholesterol + NADP+ in lysates of HEK-293 cells expressing 11beta-HSD1, no oxidation in intact HEK293 cells expressing 11beta-HSD1 Homo sapiens 7-oxocholesterol + NADPH
-
r
7beta-hydroxycholesterol + NADP+ in lysates of HEK-293 cells expressing 11beta-HSD1, no oxidation in intact HEK293 cells expressing 11beta-HSD1 Rattus norvegicus 7-oxocholesterol + NADPH
-
r
7beta-hydroxycholesterol + NADP+ in lysates of HEK-293 cells expressing 11beta-HSD1, no oxidation in intact HEK293 cells expressing 11beta-HSD1 Mesocricetus auratus 7-oxocholesterol + NADPH
-
r
corticosterone + NADP+
-
Homo sapiens 11-dehydrocorticosterone + NADPH
-
r
corticosterone + NADP+
-
Rattus norvegicus 11-dehydrocorticosterone + NADPH
-
r
corticosterone + NADP+
-
Mesocricetus auratus 11-dehydrocorticosterone + NADPH
-
r

Synonyms

Synonyms Comment Organism
11beta-HSD1
-
Homo sapiens
11beta-HSD1
-
Rattus norvegicus
11beta-HSD1
-
Mesocricetus auratus
11beta-hydroxysteroid dehydrogenase type 1
-
Homo sapiens
11beta-hydroxysteroid dehydrogenase type 1
-
Rattus norvegicus
11beta-hydroxysteroid dehydrogenase type 1
-
Mesocricetus auratus