Protein Variants | Comment | Organism |
---|---|---|
D35A/K36R | the D35A/K36R mutant enzyme of 3beta-HSD1 has no measurable 3beta-HSD activity with NADH as the cofactor and now uses NADPH as the cofactor and NADH specificity of the wild-type 1 isomerase is completely lost | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14060 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
3beta-HSD1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NADH forms hydrogen bond with Asp35 in the NADH-wild-type 3beta-HSD1 complex | Homo sapiens | |
NADP+ | NADPH forms hydrogen bond with Lys36 in the NADPH-wild-type 3beta-HSD1 complex. The total contribution of Asp35 and Lys36 is most favorable in the case of NADPH binding to the wild-type enzyme when compared with NADH, and Lys36 have strong electronic interaction with NADPH | Homo sapiens |