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Literature summary for 1.1.1.145 extracted from

  • Pletnev, V.Z.; Thomas, J.L.; Rhaney, F.L.; Holt, L.S.; Scaccia, L.A.; Umland, T.C.; Duax, W.L.
    Rational proteomics V: structure-based mutagenesis has revealed key residues responsible for substrate recognition and catalysis by the dehydrogenase and isomerase activities in human 3beta-hydroxysteroid dehydrogenase/isomerase type 1 (2006), J. Steroid Biochem. Mol. Biol., 101, 50-60.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
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Homo sapiens

Protein Variants

Protein Variants Comment Organism
E126L site-directed mutagenesis, functional characterization Homo sapiens
H232A site-directed mutagenesis, functional characterization Homo sapiens
N100A site-directed mutagenesis, functional characterization Homo sapiens
N100S site-directed mutagenesis, functional characterization Homo sapiens
N323L site-directed mutagenesis, functional characterization Homo sapiens
S322A site-directed mutagenesis, functional characterization Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is essential for biosynthesis of all classes of steroid hormones catalyzing the first step of the multienzyme pathway conversion of circulating dehydroepiandrosterone and pregnenolone to active steroid hormones ?
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens P14060 3beta-HSD1; type 1 isozyme
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Reaction

Reaction Comment Organism Reaction ID
a 3beta-hydroxy-DELTA5-steroid + NAD+ = a 3-oxo-DELTA5-steroid + NADH + H+ substrate binding and active site residues, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is essential for biosynthesis of all classes of steroid hormones catalyzing the first step of the multienzyme pathway conversion of circulating dehydroepiandrosterone and pregnenolone to active steroid hormones Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer three-dimensional structure modeling of enzyme in ternary complex with NAD+ and androstenedione using crystals structures of enzymes from other organisms, substrate binding and active site residue determination, overview Homo sapiens

Synonyms

Synonyms Comment Organism
3beta-HSD_1
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Homo sapiens
3beta-hydroxysteroid dehydrogenase/isomerase type 1
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Homo sapiens
More the enzyme belongs to the short chain dehydrogenase/reductase family Homo sapiens