Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, transient and stable expression of the virus enzyme in Epithelioma papulosum cyprini cells, expression as EGFP-fusion protein exclusively in host cell mitochondria, enzyme overexpression does not influence viral replication or cell growth, but does suppress virus-induced cytopathic effects | Rana grylio iridovirus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DELTA5-3beta-hydroxysteroid + NAD+ | Rana grylio iridovirus | the enzyme is essential for biosynthesis of all classes of steroid hormones | DELTA4-3-oxosteroid + NADH + H+ | - |
? | |
DELTA5-3beta-hydroxysteroid + NAD+ | Rana grylio iridovirus 9506 | the enzyme is essential for biosynthesis of all classes of steroid hormones | DELTA4-3-oxosteroid + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rana grylio iridovirus | Q0G9Z6 | i.e. RGV, 9506 | - |
Rana grylio iridovirus 9506 | Q0G9Z6 | i.e. RGV, 9506 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DELTA5-3beta-hydroxysteroid + NAD+ | the enzyme is essential for biosynthesis of all classes of steroid hormones | Rana grylio iridovirus | DELTA4-3-oxosteroid + NADH + H+ | - |
? | |
DELTA5-3beta-hydroxysteroid + NAD+ | the enzyme performs the 3beta-hydroxysteroid dehydrogenase and DELTA5-DELTA4 isomerase reactions | Rana grylio iridovirus | DELTA4-3-oxosteroid + NADH + H+ | - |
? | |
DELTA5-3beta-hydroxysteroid + NAD+ | the enzyme is essential for biosynthesis of all classes of steroid hormones | Rana grylio iridovirus 9506 | DELTA4-3-oxosteroid + NADH + H+ | - |
? | |
DELTA5-3beta-hydroxysteroid + NAD+ | the enzyme performs the 3beta-hydroxysteroid dehydrogenase and DELTA5-DELTA4 isomerase reactions | Rana grylio iridovirus 9506 | DELTA4-3-oxosteroid + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3beta-HSD | - |
Rana grylio iridovirus |
3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4 isomerase | - |
Rana grylio iridovirus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rana grylio iridovirus |