Inhibitors | Comment | Organism | Structure |
---|---|---|---|
17alpha-hydroxypregnenolone | competitive inhibition | Bos taurus | |
diethyldicarbonate | - |
Bos taurus | |
p-chloromercuribenzoate | - |
Bos taurus | |
pregnenolone | competitive inhibition | Bos taurus | |
trilostane | i.e. 4alpha,5-epoxy-3,17beta-dihydroxy-5alpha-androst-2-en-2-carbonitrile | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
pregnenolone | - |
Bos taurus | |
0.0053 | - |
3beta,17alpha-dihydroxy-5-pregen-20-one | - |
Bos taurus | |
0.0062 | 0.0066 | NAD+ | - |
Bos taurus | |
4.9 | - |
NADP+ | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
x * 41000, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pregnenolone + NAD(P)+ | Bos taurus | - |
pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.677 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-80°C, 0.1 M potassium phosphate buffer, pH 7.4, 10 mg protein/ml, at least 3 months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+ | - |
Bos taurus | 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H | - |
? | |
pregnenolone + NAD(P)+ | - |
Bos taurus | pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, SDS-PAGE | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.5 | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cysteinyl and histidyl at NAD+-binding site | Bos taurus | |
NADP+ | low activity | Bos taurus |