Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris and Arabidopsis thaliana | Haloarcula marismortui |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | activity is increased by nearly 120% when NaCl concentration is 2 M. Transgenic Arabidopsis thaliana ectopic expressing the enzyme (HmS6PDH) accumulates more sorbitol under salt stress | Haloarcula marismortui |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
SDS-PAGE | Haloarcula marismortui |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 6-phosphate + NADH + H+ | Haloarcula marismortui | - |
D-sorbitol 6-phosphate + NAD+ | - |
? | |
D-fructose 6-phosphate + NADH + H+ | Haloarcula marismortui ATCC 43049 | - |
D-sorbitol 6-phosphate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula marismortui | AAV45891.1 | - |
- |
Haloarcula marismortui ATCC 43049 | AAV45891.1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula marismortui |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 6-phosphate + NADH + H+ | - |
Haloarcula marismortui | D-sorbitol 6-phosphate + NAD+ | - |
? | |
D-fructose 6-phosphate + NADH + H+ | the recombinant enzyme (HmS6PDH) has no activity to glucose or sorbitol | Haloarcula marismortui | D-sorbitol 6-phosphate + NAD+ | - |
? | |
D-fructose 6-phosphate + NADH + H+ | - |
Haloarcula marismortui ATCC 43049 | D-sorbitol 6-phosphate + NAD+ | - |
? | |
D-fructose 6-phosphate + NADH + H+ | the recombinant enzyme (HmS6PDH) has no activity to glucose or sorbitol | Haloarcula marismortui ATCC 43049 | D-sorbitol 6-phosphate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, SDS-PAGE | Haloarcula marismortui |
Synonyms | Comment | Organism |
---|---|---|
D-sorbitol-6-phosphate 2-dehydrogenase | - |
Haloarcula marismortui |
HmSorD | - |
Haloarcula marismortui |
S6PDH | - |
Haloarcula marismortui |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Haloarcula marismortui |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | 50°C: about 50% of maximal activity, 70°C: about 30% of maximal activity | Haloarcula marismortui |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Haloarcula marismortui |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 5.5 | pH 2.5: about 40% of maximal activity, pH 5.5: about 45% of maximal activity | Haloarcula marismortui |