Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris, gene cloning in Escherichia coli | Agaricus bisporus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, SDS-PAGE | Agaricus bisporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NADP+ | Agaricus bisporus | - |
D-fructose + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Agaricus bisporus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | stage 2, highest activity in salt stressed fruit bodies | Agaricus bisporus | - |
mycelium | - |
Agaricus bisporus | - |
Storage Stability | Organism |
---|---|
-80°C, 50 mM MOPS, pH 7.5, 1 mM DTT, 50 mM NaCl, several weeks | Agaricus bisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NADP+ | - |
Agaricus bisporus | D-fructose + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Agaricus bisporus |
Synonyms | Comment | Organism |
---|---|---|
NADP-mannitol dehydrogenase | - |
Agaricus bisporus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for NADP+ | Agaricus bisporus |