Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptococcus sp. |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 12.5% (v/v) 2-methyl-2,4-pentanediol, 0.02 M of each carboxylic acid (0.2 M sodium formate, 0.2 M ammonium acetate, 0.2 M trisodium citrate, 0.2 M sodium potassium L-tartrate, 0.2 M sodium oxamate) and 0.1 M MES/imidazole pH 6.5 | Streptococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
dTDP-alpha-D-glucose | at pH 7.5, temperature not specified in the publication | Streptococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require metal for its activity | Streptococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
1 * 27500, SDS-PAGE | Streptococcus sp. |
32000 | - |
- |
Streptococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-beta-L-rhamnose + NADP+ | Streptococcus sp. | - |
dTDP-4-dehydro-beta-L-rhamnose + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 75 gel filtration | Streptococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-alpha-D-glucose + NADP+ | - |
Streptococcus sp. | ? | - |
? | |
dTDP-beta-L-rhamnose + NADP+ | - |
Streptococcus sp. | dTDP-4-dehydro-beta-L-rhamnose + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27500, SDS-PAGE | Streptococcus sp. |
monomer | 1 * 32000, calculated from amino acid sequence | Streptococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
dTDP-4-dehydrorhamnose reductase | - |
Streptococcus sp. |
gacA | - |
Streptococcus sp. |
RmlD | - |
Streptococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Streptococcus sp. |