Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.133 extracted from

  • Watt, G.; Leoff, C.; Harper, A.D.; Bar-Peled, M.
    A bifunctional 3,5-epimerase/4-keto reductase for nucleotide-rhamnose synthesis in Arabidopsis (2004), Plant Physiol., 134, 1337-1346.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: MgCl2, CaCl2, EDTA at 10mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
NADPH pH 8.5, 30°C Arabidopsis thaliana
0.0169
-
dTDP-4-keto-6-deoxy-D-glucose pH 8.5, 30°C Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE Arabidopsis thaliana
35500
-
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LQ04 bifunctional 3,5-epimerase/4-keto reductase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information present in all tissues examined Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-

Storage Stability

Storage Stability Organism
-20°C, repeated freezing and thawing, loss of more than 60% of activity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+ 40% of the rate with NADPH Arabidopsis thaliana dTDP-beta-L-rhamnose + NAD+
-
?
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
Arabidopsis thaliana dTDP-beta-L-rhamnose + NADP+
-
?
additional information no substrate: GDP-4-keto-6-deoxy-mannose, UDP-glucose, dTDP-glucose, UDP-xylose, GDP-fucose Arabidopsis thaliana ?
-
?
UDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
Arabidopsis thaliana UDP-beta-L-rhamnose + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45
-
about 35% of maximum activity Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADH
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana