Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Ectocarpus siliculosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | weak inhibitor of reaction | Ectocarpus siliculosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.086 | - |
NAD+ | pH and temperature not specified in the publication | Ectocarpus siliculosus | |
0.095 | - |
GDP-D-mannose | pH and temperature not specified in the publication | Ectocarpus siliculosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | at 1 mM CoCl2 44% enzymatic activity | Ectocarpus siliculosus | |
CuSO4 | at 1 mM CuSO4 no enzymatic activity | Ectocarpus siliculosus | |
FeCl3 | at 1 mM FeCl3 49% enzymatic activity | Ectocarpus siliculosus | |
FeSO4 | at 1 mM FeSO4 77% enzymatic activity | Ectocarpus siliculosus | |
K+ | increases activity | Ectocarpus siliculosus | |
Na+ | increases activity | Ectocarpus siliculosus | |
PCMB | 0.025 mM, 0.8% activity | Ectocarpus siliculosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
SDS-PAGE | Ectocarpus siliculosus |
35000 | - |
2 * 35000, gel filtration | Ectocarpus siliculosus |
70000 | - |
gel filtration | Ectocarpus siliculosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ectocarpus siliculosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Ectocarpus siliculosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-D-mannose + NAD+ + H2O | - |
Ectocarpus siliculosus | GDP-D-mannuronate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, gel filtration | Ectocarpus siliculosus |
Synonyms | Comment | Organism |
---|---|---|
GDP-mannose dehydrogenase | - |
Ectocarpus siliculosus |
GMD | - |
Ectocarpus siliculosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
enzyme partly inactivated above 30°C | Ectocarpus siliculosus |
45 | - |
enzyme fully inactivated at 45°C after 15 min | Ectocarpus siliculosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.75 | - |
assay at | Ectocarpus siliculosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.75 | 9 | optimal pH range, enzyme inactive below pH 7 | Ectocarpus siliculosus |