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Literature summary for 1.1.1.132 extracted from

  • Tenhaken, R.; Voglas, E.; Cock, J.M.; Neu, V.; Huber, C.G.
    Characterization of GDP-mannose dehydrogenase from the brown alga Ectocarpus siliculosus providing the precursor for the alginate polymer (2011), J. Biol. Chem., 286, 16707-16715.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Ectocarpus siliculosus

Inhibitors

Inhibitors Comment Organism Structure
NADPH weak inhibitor of reaction Ectocarpus siliculosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.086
-
NAD+ pH and temperature not specified in the publication Ectocarpus siliculosus
0.095
-
GDP-D-mannose pH and temperature not specified in the publication Ectocarpus siliculosus

Metals/Ions

Metals/Ions Comment Organism Structure
CoCl2 at 1 mM CoCl2 44% enzymatic activity Ectocarpus siliculosus
CuSO4 at 1 mM CuSO4 no enzymatic activity Ectocarpus siliculosus
FeCl3 at 1 mM FeCl3 49% enzymatic activity Ectocarpus siliculosus
FeSO4 at 1 mM FeSO4 77% enzymatic activity Ectocarpus siliculosus
K+ increases activity Ectocarpus siliculosus
Na+ increases activity Ectocarpus siliculosus
PCMB 0.025 mM, 0.8% activity Ectocarpus siliculosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
SDS-PAGE Ectocarpus siliculosus
35000
-
2 * 35000, gel filtration Ectocarpus siliculosus
70000
-
gel filtration Ectocarpus siliculosus

Organism

Organism UniProt Comment Textmining
Ectocarpus siliculosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Ectocarpus siliculosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-D-mannose + NAD+ + H2O
-
Ectocarpus siliculosus GDP-D-mannuronate + NADH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000, gel filtration Ectocarpus siliculosus

Synonyms

Synonyms Comment Organism
GDP-mannose dehydrogenase
-
Ectocarpus siliculosus
GMD
-
Ectocarpus siliculosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
enzyme partly inactivated above 30°C Ectocarpus siliculosus
45
-
enzyme fully inactivated at 45°C after 15 min Ectocarpus siliculosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.75
-
assay at Ectocarpus siliculosus

pH Range

pH Minimum pH Maximum Comment Organism
8.75 9 optimal pH range, enzyme inactive below pH 7 Ectocarpus siliculosus