Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 72% inhibition at 0.01 mM | Dickeya chrysanthemi | |
ATP | 30% inhibition for both reduction and oxidation reaction at 1 mM | Dickeya chrysanthemi | |
p-chloromercuribenzoate | 50% inhibition at 0.02 mM | Dickeya chrysanthemi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADH | - |
Dickeya chrysanthemi | |
0.34 | - |
(4S)-4,6-dihydroxy-2,5-dioxohexanoate | - |
Dickeya chrysanthemi | |
0.4 | - |
NAD+ | - |
Dickeya chrysanthemi | |
7.7 | - |
2-dehydro-3-deoxy-D-gluconate | - |
Dickeya chrysanthemi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 20% activation at 1 mM | Dickeya chrysanthemi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-D-gluconate + NAD+ | Dickeya chrysanthemi | involved in pectinolysis by some phytopathogenic bacteria | (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dickeya chrysanthemi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, 2step chromatography | Dickeya chrysanthemi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Dickeya chrysanthemi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
39 | - |
- |
Dickeya chrysanthemi |
Storage Stability | Organism |
---|---|
-20°C, 2 mM DTT, 6 months | Dickeya chrysanthemi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-D-gluconate + NAD+ | involved in pectinolysis by some phytopathogenic bacteria | Dickeya chrysanthemi | (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH | - |
r | |
pyruvate + NAD+ | 10% of the activity compared to 2-dehydro-3-deoxy-D-gluconate | Dickeya chrysanthemi | ? + NADH | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Dickeya chrysanthemi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
stable for 15 h | Dickeya chrysanthemi |
46 | - |
half-life: 10 min, NADH increases thermal stability | Dickeya chrysanthemi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | reduction reaction | Dickeya chrysanthemi |
10 | - |
oxidation reaction | Dickeya chrysanthemi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Dickeya chrysanthemi | |
NADH | - |
Dickeya chrysanthemi | |
NADP+ | 53% lower activity than with NAD+ | Dickeya chrysanthemi | |
NADPH | 62% lower activity than with NADH | Dickeya chrysanthemi |