Crystallization (Comment) | Organism |
---|---|
in complex with NAD+, sitting drop vapor diffusion method, using 0.1 M bicine pH 9.0, 2% (w/v) dioxane, 10% (w/v) PEG 20 000 | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-D-gluconate + NAD+ | Thermus thermophilus | - |
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+ | - |
? | |
2-dehydro-3-deoxy-D-gluconate + NAD+ | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Super Q Toyopearl 650M column chromatography, Resource Q column chromatography, Bio-Scale CHT20-I column chromatography, and Superdex 75 gel filtration | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-D-gluconate + NAD+ | - |
Thermus thermophilus | (4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+ | - |
? | |
2-dehydro-3-deoxy-D-gluconate + NAD+ | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | (4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | X-ay crystallography | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
DDGDH | - |
Thermus thermophilus |
NAD-dependent 3-dehydro-2-deoxy-D-gluconate dehydrogenase | - |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | this enzyme prefers NAD+ as the physiological cofactor | Thermus thermophilus |