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Literature summary for 1.1.1.124 extracted from

  • Schrimsher, J.L.; Wingfield, P.T.; Bernard, A.; Mattaliano, R.; Payton, M.A.
    Purification and characterization of 5-ketofructose reductase from Erwinia citreus (1988), Biochem. J., 253, 511-516.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
D-fructose non-competitive inhibition Erwinia sp.
NADP+ competitive inhibition of D-fructose synthesis, non-competitive inhibition of 5-keto-fructose synthesis Erwinia sp.
NADPH competitive inhibition of D-fructose oxidation Erwinia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
NADP+
-
Erwinia sp.
0.071
-
NADPH
-
Erwinia sp.
0.32
-
NADH
-
Erwinia sp.
1.97
-
NAD+
-
Erwinia sp.
9.1
-
D-fructose
-
Erwinia sp.
12.7
-
5-keto-D-fructose
-
Erwinia sp.
140
-
D-arabinose
-
Erwinia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
1 * 40000, SDS-PAGE Erwinia sp.
50000
-
gel filtration, sedimentation equilibrium measurement Erwinia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose + NADP+ Erwinia sp. highly specific for D-fructose 5-dehydro-D-fructose + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Erwinia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Erwinia sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Erwinia sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2450
-
-
Erwinia sp.

Storage Stability

Storage Stability Organism
-20°C, 30 mM Tris-HCl, pH 7.8, 3 months Erwinia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-dehydro-D-fructose + NADH + H+
-
Erwinia sp. D-fructose + NAD+
-
r
D-arabinose + NADP+ 5fold lower activity than for D-fructose Erwinia sp. ? + NADPH
-
?
D-fructose + NAD+
-
Erwinia sp. 5-dehydro-D-fructose + NADH + H+
-
r
D-fructose + NADP+ highly specific for D-fructose Erwinia sp. 5-dehydro-D-fructose + NADPH
-
r

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Erwinia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reduction of 5-keto-D-fructose Erwinia sp.
9.5
-
oxidation of D-fructose Erwinia sp.

Cofactor

Cofactor Comment Organism Structure
NADH substitutes for NADPH Erwinia sp.
NADPH oxidized and reduced cofactors bind to the same site of the enzyme Erwinia sp.