Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Acinetobacter sp. | |
Co2+ | complete inhibition at 1 mM | Acinetobacter sp. | |
Cu2+ | complete inhibition at 1 mM | Acinetobacter sp. | |
EDTA | - |
Acinetobacter sp. | |
Hg2+ | complete inhibition at 1 mM | Acinetobacter sp. | |
N-ethylmaleimide | - |
Acinetobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
NADP+ | at pH 8.0 and 37°C | Acinetobacter sp. | |
2.6 | - |
L-fucose | at pH 8.0 and 37°C | Acinetobacter sp. | |
2.7 | - |
NAD+ | at pH 8.0 and 37°C | Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
gel filtration | Acinetobacter sp. |
25000 | - |
1 * 25000, SDS-PAGE | Acinetobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-fucose + NADP+ | Acinetobacter sp. | 100% activity | L-fuconolactone + NADPH + H+ | - |
? | |
L-fucose + NADP+ | Acinetobacter sp. SA-134 | 100% activity | L-fuconolactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Acinetobacter sp. SA-134 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, phenyl-Toyopearl column chromatography, Q-Sepharose column chromatography, Superdex 200HR gel filtration, and Mono Q column chromatography | Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.3 | - |
cell-free extract, at pH 8.0 and 37°C | Acinetobacter sp. |
217 | - |
after 94.3fold purification, at pH 8.0 and 37°C | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + NADP+ | good substrate with 65% activity compared to L-fucose | Acinetobacter sp. | D-arabinolactone + NADPH + H+ | - |
? | |
D-arabinose + NADP+ | good substrate with 65% activity compared to L-fucose | Acinetobacter sp. SA-134 | D-arabinolactone + NADPH + H+ | - |
? | |
L-fucose + NAD+ | - |
Acinetobacter sp. | L-fuconolactone + NADH + H+ | - |
? | |
L-fucose + NAD+ | - |
Acinetobacter sp. SA-134 | L-fuconolactone + NADH + H+ | - |
? | |
L-fucose + NADP+ | good substrate | Acinetobacter sp. | L-fuconolactone + NADPH + H+ | - |
? | |
L-fucose + NADP+ | 100% activity | Acinetobacter sp. | L-fuconolactone + NADPH + H+ | - |
? | |
L-fucose + NADP+ | good substrate | Acinetobacter sp. SA-134 | L-fuconolactone + NADPH + H+ | - |
? | |
L-fucose + NADP+ | 100% activity | Acinetobacter sp. SA-134 | L-fuconolactone + NADPH + H+ | - |
? | |
L-galactose + NADP+ | L-galactose is a poor substrate with 11% activity compared to L-fucose | Acinetobacter sp. | ? | - |
? | |
additional information | no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose | Acinetobacter sp. | ? | - |
? | |
additional information | no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose | Acinetobacter sp. SA-134 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25000, SDS-PAGE | Acinetobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
L-fucose dehydrogenase | - |
Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Acinetobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
the enzyme is stable below 35°C for 60 min | Acinetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Acinetobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the enzyme is stable between pH 6.0 and 10.0 for 60 min | Acinetobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme acts on both NAD+ and NADP+ in similar manner | Acinetobacter sp. | |
NADP+ | the enzyme acts on both NAD+ and NADP+ in similar manner | Acinetobacter sp. |