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Literature summary for 1.1.1.121 extracted from

  • Asada, Y.; Endo, S.; Inoue, Y.; Mamiya, H.; Hara, A.; Kunishima, N.; Matsunaga, T.
    Biochemical and structural characterization of a short-chain dehydrogenase/reductase of Thermus thermophilus HB8. A hyperthermostable aldose-1-dehydrogenase with broad substrate specificity (2009), Chem. Biol. Interact., 178, 117-126 .
    View publication on PubMed
Search for more literature for 1.1.1.121

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
oil-microbatch method, crystal structure of the enzyme-NAD+ binary complex at 1.65 A resolution. The structure provides evidence for the strict coenzyme specificity and broad substrate specificity of the enzyme Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
D242N mutation results in a large decrease in thermostability without dissociation into dimer or monomer Thermus thermophilus
F141A mutation results in decrease in thermostability without dissociation into dimer or monomer Thermus thermophilus
F249A mutation results in decrease in thermostability without dissociation into dimer or monomer Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline competitive inhibition Thermus thermophilus
2,3-benzofuran competitive inhibition Thermus thermophilus
Hexestrol competitive inhibition Thermus thermophilus
indole competitive inhibition Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 1-Acenaphthenol pH 7.4, 50°C Thermus thermophilus
0.17
-
 (S)-tetralol pH 7.4, 50°C Thermus thermophilus
0.25
-
 4-chromanol pH 7.4, 50°C Thermus thermophilus
0.65
-
 (S)-indanol pH 7.4, 50°C Thermus thermophilus
1.3
-
 (R)-indanol pH 7.4, 50°C Thermus thermophilus
2 3 D-galactosamine pH 7.4, 50°C Thermus thermophilus
2.5
-
 (R)-tetralol pH 7.4, 50°C Thermus thermophilus
3.7
-
 2-Cyclohexen-1-ol pH 7.4, 50°C Thermus thermophilus
5.5
-
 D-fucose pH 7.4, 50°C Thermus thermophilus
27
-
 cis-benzene dihydrodiol pH 7.4, 50°C Thermus thermophilus
33
-
 2-deoxy-D-galactose pH 7.4, 50°C Thermus thermophilus
78
-
 D-galactose pH 7.4, 50°C Thermus thermophilus
131
-
 D-talose pH 7.4, 50°C Thermus thermophilus
139
-
 D-glucosamine pH 7.4, 50°C Thermus thermophilus
162
-
 L-fucose pH 7.4, 50°C Thermus thermophilus
178
-
 6-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
250
-
 cellobiose pH 7.4, 50°C Thermus thermophilus
262
-
 L-arabinose pH 7.4, 50°C Thermus thermophilus
300
-
 2-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
580
-
 D-glucose pH 7.4, 50°C Thermus thermophilus
781
-
 lactose pH 7.4, 50°C Thermus thermophilus
841
-
 D-xylose pH 7.4, 50°C Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
 gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SLC4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-indanol + NAD+
-
 Thermus thermophilus 2,3-dihydro-1H-inden-1-one + NADH + H+
-
 ?
(R)-tetralol + NAD+
-
 Thermus thermophilus 3,4-dihydronaphthalen-2(1H)-one + NADH + H+
-
 ?
(S)-indanol + NAD+
-
 Thermus thermophilus 2,3-dihydro-1H-inden-1-one + NADH + H+
-
 ?
(S)-tetralol + NAD+
-
 Thermus thermophilus 3,4-dihydronaphthalen-2(1H)-one + NADH + H+
-
 ?
1-acenaphthenol + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
2-cyclohexen-1-ol + NAD+
-
 Thermus thermophilus 2-cyclohexen-1-one + NADH + H+
-
 ?
2-deoxy-D-galactose + NAD+
-
 Thermus thermophilus 2-deoxy-D-galactono-delta-lactone + NADH + H+
-
 ?
2-deoxy-D-glucose + NAD+
-
 Thermus thermophilus 2-deoxy-D-glucono-delta-lactone + NADH + H+
-
 ?
4-chromanol + NAD+
-
 Thermus thermophilus 2,3-dihydro-4H-chromen-4-one + NADH + H+
-
 ?
6-deoxy-D-glucose + NAD+
-
 Thermus thermophilus 6-deoxy-D-glucono-delta-lactone + NADH + H+
-
 ?
cellobiose + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
cis-benzene dihydrodiol + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
D-fucose + NAD+
-
 Thermus thermophilus D-fucono-delta-lactone + NADH + H+
-
 ?
D-galactosamine + NAD+
-
 Thermus thermophilus D-galactosamino-delta-lactone + NADH + H+
-
 ?
D-galactose + NAD+
-
 Thermus thermophilus D-galactono-delta-lactone + NADH + H+
-
 ?
D-glucosamine + NAD+
-
 Thermus thermophilus D-glucosamino-delta-lactone + NADH + H+
-
 ?
D-glucose + NAD+
-
 Thermus thermophilus D-glucono-delta-lactone + NADH + H+
-
 ?
D-talose + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
D-xylose + NAD+
-
 Thermus thermophilus D-xylono-delta-lactone + NADH + H+
-
 ?
L-arabinose + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
L-fucose + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
lactose + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 27000, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
aldose-1-dehydrogenase
-
 Thermus thermophilus
TTHA0369
-
 Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 mutant enzyme D242N loses about 20% of activity Thermus thermophilus
85
-
 15 min, wild-type enzyme is stable, mutant enzyme F141A loses about 5% of activity, mutant enzyme F249A loses about 10% of activity, mutant enzyme D242N loses 60% of activity Thermus thermophilus
90
-
 15 min, wild-type enzyme loses about 10% of its activity, mutant enzyme F141A loses about 25% of activity, mutant enzyme F249A loses about 25% of activity, mutant enzyme D242N completely loses activity Thermus thermophilus
95
-
 15 min, wild-type enzyme loses about 40% of its activity, mutant enzyme F141A loses about 50% of activity, mutant enzyme F249A loses about 70% of activity, mutant enzyme D242N completely loses activity Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.075
-
 (R)-tetralol pH 7.4, 50°C Thermus thermophilus
0.11
-
 L-fucose pH 7.4, 50°C Thermus thermophilus
0.17
-
 (R)-indanol pH 7.4, 50°C Thermus thermophilus
0.31
-
 D-galactosamine pH 7.4, 50°C Thermus thermophilus
0.42
-
 D-xylose pH 7.4, 50°C Thermus thermophilus
0.62
-
 D-glucosamine pH 7.4, 50°C Thermus thermophilus
3
-
 D-talose pH 7.4, 50°C Thermus thermophilus
3.5
-
 2-Cyclohexen-1-ol pH 7.4, 50°C Thermus thermophilus
3.85
-
 D-glucose pH 7.4, 50°C Thermus thermophilus
4.55
-
 L-arabinose pH 7.4, 50°C Thermus thermophilus
5.77
-
 cellobiose pH 7.4, 50°C Thermus thermophilus
6.35
-
 6-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
6.9
-
 2-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
7.4
-
 cis-benzene dihydrodiol pH 7.4, 50°C Thermus thermophilus
8.25
-
 D-galactose pH 7.4, 50°C Thermus thermophilus
8.7
-
 D-fucose pH 7.4, 50°C Thermus thermophilus
9.23
-
 lactose pH 7.4, 50°C Thermus thermophilus
12.8
-
 2-deoxy-D-galactose pH 7.4, 50°C Thermus thermophilus
40.3
-
 4-chromanol pH 7.4, 50°C Thermus thermophilus
138.7
-
 (S)-indanol pH 7.4, 50°C Thermus thermophilus
161.7
-
 (S)-tetralol pH 7.4, 50°C Thermus thermophilus
4333
-
 1-Acenaphthenol pH 7.4, 50°C Thermus thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 13 50°C, 30 min, stable Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0005
-
 D-xylose pH 7.4, 50°C Thermus thermophilus
0.00067
-
 L-fucose pH 7.4, 50°C Thermus thermophilus
0.0045
-
 D-glucosamine pH 7.4, 50°C Thermus thermophilus
0.0067
-
 D-glucose pH 7.4, 50°C Thermus thermophilus
0.012
-
 lactose pH 7.4, 50°C Thermus thermophilus
0.014
-
 D-galactosamine pH 7.4, 50°C Thermus thermophilus
0.017
-
 L-arabinose pH 7.4, 50°C Thermus thermophilus
0.023
-
 cellobiose pH 7.4, 50°C Thermus thermophilus
0.023
-
 2-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
0.023
-
 D-talose pH 7.4, 50°C Thermus thermophilus
0.03
-
 (R)-tetralol pH 7.4, 50°C Thermus thermophilus
0.035
-
 6-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
0.11
-
 D-galactose pH 7.4, 50°C Thermus thermophilus
0.13
-
 (R)-indanol pH 7.4, 50°C Thermus thermophilus
0.27
-
 cis-benzene dihydrodiol pH 7.4, 50°C Thermus thermophilus
0.38
-
 2-deoxy-D-galactose pH 7.4, 50°C Thermus thermophilus
0.95
-
 2-Cyclohexen-1-ol pH 7.4, 50°C Thermus thermophilus
1.58
-
 D-fucose pH 7.4, 50°C Thermus thermophilus
61.7
-
 4-chromanol pH 7.4, 50°C Thermus thermophilus
216.7
-
 (S)-indanol pH 7.4, 50°C Thermus thermophilus
950
-
 (S)-tetralol pH 7.4, 50°C Thermus thermophilus
3000
-
 1-Acenaphthenol pH 7.4, 50°C Thermus thermophilus