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Literature summary for 1.1.1.121 extracted from
- Analysis of bacterial glucose dehydrogenase homologs from thermoacidophilic archaeon Thermoplasma acidophilum: finding and characterization of aldohexose dehydrogenase (2004), Biosci. Biotechnol. Biochem., 68, 2451-2456.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Thermoplasma acidophilum |  |
| Cu2+ | - |
Thermoplasma acidophilum | |
| Hg2+ | - |
Thermoplasma acidophilum | |
| Ni2+ | - |
Thermoplasma acidophilum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 24.3 | - |
2-deoxy-D-glucose | pH 8.0, 37°C | Thermoplasma acidophilum | |
| 25 | - |
D-mannose | pH 8.0, 37°C | Thermoplasma acidophilum | |
| 208 | - |
D-glucose | pH 8.0, 37°C | Thermoplasma acidophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, SDS-PAGE | Thermoplasma acidophilum |
| 85000 | - |
gel filtration | Thermoplasma acidophilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoplasma acidophilum | Q9HK51 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
pH 8.0, 37°C, substrates: D-glucose + NAD+ | Thermoplasma acidophilum |
| 15 | - |
pH 8.0, 37°C, substrates: D-xylose + NAD+ | Thermoplasma acidophilum |
| 24 | - |
pH 8.0, 37°C, substrates: 2-deoxy-D-glucose + NAD+ | Thermoplasma acidophilum |
| 31 | - |
pH 8.0, 37°C, substrates: D-mannose + NAD+ | Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-glucose + NAD+ | relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+ | Thermoplasma acidophilum | 2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| 2-deoxy-D-glucose + NADP+ | relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+ | Thermoplasma acidophilum | 2-deoxy-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
| D-glucose + NAD+ | activity is 23% compared to the activity with D-mannose and NAD+. No activity with beta-D-glucose + NADP+ | Thermoplasma acidophilum | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| D-mannose + NAD+ | relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+ | Thermoplasma acidophilum | D-mannono-1,5-lactone + NADH | - |
? | |
| D-mannose + NADP+ | relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+ | Thermoplasma acidophilum | D-mannono-1,5-lactone + NADPH | - |
? | |
| D-xylose + NAD+ | relative activity with D-xylose and NADP+ is less than 5% compared to the activity with D-xylose and NAD+ | Thermoplasma acidophilum | D-xylono-1,5-lactone + NADH + H+ | - |
? | |
| D-xylose + NADP+ | relative activity with D-xylose and NADP+ is less than 5% compared to the activity with D-xylose and NAD+ | Thermoplasma acidophilum | D-xylono-1,5-lactone + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Thermoplasma acidophilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aldohexose dehydrogenase | - |
Thermoplasma acidophilum |
| Ta0754 | - |
Thermoplasma acidophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Thermoplasma acidophilum |
| 70 | - |
- |
Thermoplasma acidophilum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to, pH 7.5, 15 min | Thermoplasma acidophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Thermoplasma acidophilum |
| 10 | - |
- |
Thermoplasma acidophilum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | 25°C, 16 h, no loss of activity | Thermoplasma acidophilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+. Relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+ | Thermoplasma acidophilum | |
| NADP+ | relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+. Relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+ | Thermoplasma acidophilum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermoplasma acidophilum | isoelectric focusing | - |
5.2 |
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