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Literature summary for 1.1.1.120 extracted from

  • Aro-Kaerkkaeinen, N.; Toivari, M.; Maaheimo, H.; Ylilauri, M.; Pentikaeinen, O.T.; Andberg, M.; Oja, M.; Penttilae, M.; Wiebe, M.G.; Ruohonen, L.; Koivula, A.
    L-arabinose/D-galactose 1-dehydrogenase of Rhizobium leguminosarum bv. trifolii characterised and applied for bioconversion of L-arabinose to L-arabonate with Saccharomyces cerevisiae (2014), Appl. Microbiol. Biotechnol., 98, 9653-9665.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain H3756 Rhizobium leguminosarum bv. trifolii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
 D-galactose with NAD+ as cosubstrate, at pH 9.0 and 23°C Rhizobium leguminosarum bv. trifolii
0.5
-
 D-galactose with NADP+ as cosubstrate, at pH 9.0 and 23°C Rhizobium leguminosarum bv. trifolii

Organism

Organism UniProt Comment Textmining
Rhizobium leguminosarum bv. trifolii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fucose + NAD+
-
 Rhizobium leguminosarum bv. trifolii ?
-
 ?
D-fucose + NADP+ 100% activity Rhizobium leguminosarum bv. trifolii ?
-
 ?
D-galactose + NAD+
-
 Rhizobium leguminosarum bv. trifolii D-galactono-1,5-lactone + NADH + H+
-
 ?
D-galactose + NADP+ 69% activity compared to D-fucose Rhizobium leguminosarum bv. trifolii D-galactono-1,5-lactone + NADPH + H+
-
 ?
L-arabinose + NAD+
-
 Rhizobium leguminosarum bv. trifolii L-arabino-1,5-lactone + NADH + H+
-
 ?
L-arabinose + NADP+ 12% activity compared to D-fucose Rhizobium leguminosarum bv. trifolii ?
-
 ?
L-arabinose + NADP+ 21% activity compared to D-fucose Rhizobium leguminosarum bv. trifolii L-arabino-1,5-lactone + NADPH + H+
-
 ?
additional information some minor activity (about 1%) can be measured with D-xylose and L-lyxose, while no activity is detected with D-glucose or L-rhamnose Rhizobium leguminosarum bv. trifolii ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 000, SDS-PAGE Rhizobium leguminosarum bv. trifolii

Synonyms

Synonyms Comment Organism
AraDH
-
 Rhizobium leguminosarum bv. trifolii
L-arabinose/D-galactose 1-dehydrogenase
-
 Rhizobium leguminosarum bv. trifolii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38.12
-
 D-galactose with NAD+ as cosubstrate, at pH 9.0 and 23°C Rhizobium leguminosarum bv. trifolii
69.9
-
 D-galactose with NADP+ as cosubstrate, at pH 9.0 and 23°C Rhizobium leguminosarum bv. trifolii

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme prefers NADP+ but uses also NAD+ as a cofactor Rhizobium leguminosarum bv. trifolii
NADP+ the enzyme prefers NADP+ but uses also NAD+ as a cofactor Rhizobium leguminosarum bv. trifolii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
80.17
-
 D-galactose with NAD+ as cosubstrate, at pH 9.0 and 23°C Rhizobium leguminosarum bv. trifolii
140
-
 D-galactose with NADP+ as cosubstrate, at pH 9.0 and 23°C Rhizobium leguminosarum bv. trifolii