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Literature summary for 1.1.1.12 extracted from

  • Rutten, L.; Ribot, C.; Trejo-Aguilar, B.; Woesten, H.A.; de Vries, R.P.
    A single amino acid change (Y318F) in the L-arabitol dehydrogenase (LadA) from Aspergillus niger results in a significant increase in affinity for D-sorbitol (2009), BMC Microbiol., 9, 166.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.12

Cloned(Commentary)

Cloned (Comment) Organism
derivatives of the expression vector pQE32 containing wild-type and mutated versions of ladA transformed to Escherichia coli M13 cells Aspergillus niger

Crystallization (Commentary)

Crystallization (Comment) Organism
modelling of LadA on human SDH identifies residues M70 and Y318 in LadA, that may explain the absence of activity on D-sorbitol Aspergillus niger

Protein Variants

Protein Variants Comment Organism
M70F almost complete loss of activity Aspergillus niger
M70F results in a nearly complete enzyme inactivation Aspergillus niger
Y318F results in a significant increase in affinity for D-sorbitol, xylitol and L-arabitol Aspergillus niger
Y318F increased activity for L-arabinitol and xylitol, increased affinity for D-sorbitol Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
 L-arabitol wild-type Aspergillus niger
0.056
-
 L-arabinitol wild-type, pH 9.6, 20°C Aspergillus niger
0.078
-
 L-arabitol mutant Y318F Aspergillus niger
0.078
-
 L-arabinitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
0.218
-
 xylitol mutant Y318F Aspergillus niger
0.218
-
 xylitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
0.25
-
 xylitol wild-type Aspergillus niger
0.25
-
 xylitol wild-type, pH 9.6, 20°C Aspergillus niger
0.868
-
 D-sorbitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
4.12
-
 D-sorbitol wild-type, pH 9.6, 20°C Aspergillus niger
4.122
-
 D-sorbitol wild-type Aspergillus niger
4.868
-
 D-sorbitol mutant Y318F Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger A2QAC0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins purified using Ni-agarose Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sorbitol + NAD+
-
 Aspergillus niger D-fructose + NADH + H+
-
 ?
D-sorbitol + NAD+ wild-type shows no activity on D-sorbitol, whereas mutant Y318F does Aspergillus niger ? + NADH + H+
-
 ?
L-arabinitol + NAD+
-
 Aspergillus niger L-xylulose + NADH + H+
-
 ?
L-arabitol + NAD+
-
 Aspergillus niger L-xylulose + NADH + H+
-
 ?
additional information Y318 of LadA contributes significantly to the substrate specificity difference between LAD and xylitol dehydrogenase/D-sorbitol dehydrogenase Aspergillus niger ?
-
 ?
xylitol + NAD+
-
 Aspergillus niger D-xylulose + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
L-arabitol dehydrogenase
-
 Aspergillus niger
LAD
-
 Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.48
-
 D-sorbitol wild-type, pH 9.6, 20°C Aspergillus niger
13.88
-
 D-sorbitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
14.4
-
 L-arabinitol wild-type, pH 9.6, 20°C Aspergillus niger
19.67
-
 xylitol wild-type, pH 9.6, 20°C Aspergillus niger
30
-
 L-arabinitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
36.8
-
 xylitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
809
-
 D-sorbitol wild-type Aspergillus niger
833
-
 D-sorbitol mutant Y318F Aspergillus niger
863
-
 L-arabitol wild-type Aspergillus niger
1180
-
 xylitol wild-type Aspergillus niger
1800
-
 L-arabitol mutant Y318F Aspergillus niger
2208
-
 xylitol mutant Y318F Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Aspergillus niger