Literature summary for 1.1.1.119 extracted from

Esclapez, J.; Britton, K.L.; Baker, P.J.; Fisher, M.; Pire, C.; Ferrer, J.; Bonete, M.J.; Rice, D.W.
Crystallization and preliminary X-ray analysis of binary and ternary complexes of Haloferax mediterranei glucose dehydrogenase (2005), Acta Crystallogr. Sect. F, 61, 743-746.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Zn2+	-	Haloferax mediterranei

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapour diffusion	Haloferax mediterranei
hanging-drop vapour-diffusion method at 17°C, crystallization of a range of binary and ternary complexes of the wild-type and a D38C mutant protein. For most of the complexes, crystals belonging to space group I222 are obtained using sodium/potassium citrate as a precipitant. For the binary and non-productive ternary complexes with NADPH/Zn, it is necessary to replace the citrate with 2-methyl-2,4-pentanediol. Despite the radical change in conditions, the crystals thus formed are isomorphous	Haloferax mediterranei

Crystallization (Comment)

Organism

hanging-drop vapour diffusion

Haloferax mediterranei

hanging-drop vapour-diffusion method at 17°C, crystallization of a range of binary and ternary complexes of the wild-type and a D38C mutant protein. For most of the complexes, crystals belonging to space group I222 are obtained using sodium/potassium citrate as a precipitant. For the binary and non-productive ternary complexes with NADPH/Zn, it is necessary to replace the citrate with 2-methyl-2,4-pentanediol. Despite the radical change in conditions, the crystals thus formed are isomorphous

Haloferax mediterranei

Protein Variants

Protein Variants	Comment	Organism
D38C	crystallization of a range of binary and ternary complexes of the wild-type and a D38C mutant protein	Haloferax mediterranei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
19500	-	2 * 19500	Haloferax mediterranei
39000	-	-	Haloferax mediterranei

Organism

Organism	UniProt	Comment	Textmining
Haloferax mediterranei	-	strain ATCC 33500/R4	-
Haloferax mediterranei	Q977U7	-	-
Haloferax mediterranei DSM 1411	Q977U7	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Haloferax mediterranei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-galactose + NADP+	-	Haloferax mediterranei	D-galactono-1,5-lactone + NADPH	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 19500	Haloferax mediterranei

Synonyms

Synonyms	Comment	Organism
GlcDH	-	Haloferax mediterranei
glucose dehydrogenase	-	Haloferax mediterranei

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Haloferax mediterranei