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Literature summary for 1.1.1.119 extracted from

  • Avigad, G.; Alroy, Y.; Englard, S.
    Purification and properties of a nicotinamide adenine dinucleotide phosphate-linked aldohexose dehydrogenase from Gluconobacter cerinus (1968), J. Biol. Chem., 243, 1936-1941.
    View publication on PubMed

General Stability

General Stability Organism
freezing and thawing: significant loss of activity Gluconobacter cerinus
lyophilization of dilute enzyme solutions, below 0.5 ng/ml, results in significant loss of activity Gluconobacter cerinus

Inhibitors

Inhibitors Comment Organism Structure
CuSO4
-
Gluconobacter cerinus
additional information overview: sugars found to be neither substrates nor inhibitors Gluconobacter cerinus
NADPH competitive to NADP+ Gluconobacter cerinus
ZnSO4
-
Gluconobacter cerinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
NADP+
-
Gluconobacter cerinus
0.038
-
NADPH
-
Gluconobacter cerinus
1.8
-
D-mannose in 0.1 M Tris-buffer pH 8.0 Gluconobacter cerinus
5.3
-
D-glucose
-
Gluconobacter cerinus
5.6
-
D-mannose in 0.1 M potassium phosphate pH 6.2 Gluconobacter cerinus
29
-
2-amino-2-deoxy-D-mannose
-
Gluconobacter cerinus
39
-
2-deoxy-D-glucose
-
Gluconobacter cerinus

Organism

Organism UniProt Comment Textmining
Gluconobacter cerinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Gluconobacter cerinus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
10.1
-
-
Gluconobacter cerinus

Storage Stability

Storage Stability Organism
-20┬░C, 1 year Gluconobacter cerinus
4┬░C, pH 5.4 - pH 8.5, 8 weeks Gluconobacter cerinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-2-deoxy-D-mannose + NADP+
-
Gluconobacter cerinus 2-amino-2-deoxy-D-mannono-1,5-lactone + NADPH
-
?
2-deoxy-D-glucose + NADP+
-
Gluconobacter cerinus 2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
?
D-glucose + NADP+
-
Gluconobacter cerinus D-glucono-1,5-lactone + NADPH + H+
-
r
D-mannose + NADP+
-
Gluconobacter cerinus D-mannono-1,5-lactone + NADPH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
in Tris-buffer Gluconobacter cerinus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 broad range of optimal activity, in Tris-buffer consistently higher than that in phosphate-buffers Gluconobacter cerinus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Gluconobacter cerinus
NADPH
-
Gluconobacter cerinus